Ana Martín-González

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Reactive oxygen species (ROS) induction by exposure to heavy metals (Cd, Cu or Zn) in diverse free-living ciliated protozoa (Tetrahymena sp. and three strains of Colpoda steinii, isolated from freshwater and soils with different level of metal pollution) has been evaluated. Using specific fluorophores, such as 2',7'-dichlorofluorescein diacetate,(More)
BACKGROUND Metallothioneins are ubiquitous small, cysteine-rich, multifunctional proteins which can bind heavy metals. METHODOLOGY/PRINCIPAL FINDINGS We report the results of phylogenetic and gene expression analyses that include two new Tetrahymena thermophila metallothionein genes (MTT3 and MTT5). Sequence alignments of all known Tetrahymena(More)
Laboratory toxicity tests, using ciliated protozoa, are scarce and they have been carried out usually with freshwater species. In this study, we have analysed the acute cytotoxicity of Cd, Zn and Cu in five different strains of very common soil ciliate species (Colpoda steinii, Colpoda inflata and Cyrtolophosis elongata), which were isolated from very(More)
In the present study, we have assessed by flow cytometry, the cytotoxicity of the heavy metals Cd, Zn and Cu on populations of the ciliated protozoan Tetrahymena thermophila. The obtained LC(50) for these metals was estimated as 0.195mgCdl(-1), 3.58mgZnl(-1) and 0.47mgCul(-1), respectively. As a result, the toxicity rank for this eukaryotic microorganism is(More)
This review outlines the main features of ciliate resting-cyst formation or encystment. It represents a strategy against several environmental stresses (such as starvation), which involves a highly gene-regulated cell differentiation process and originates a more resistant, differentiated form or resting cyst. This process is mainly characterized by drastic(More)
M. Speranza*, J. Wierzchos1, J. Alonso, L. Bettucci, A. Martín-González, and C. Ascaso IRN-CCMA, Department of Systems Ecology, CSIC, Serrano 115 bis, 28006, Madrid, Spain. Museum of Natural Science Álava, Siervas de Jesús, 24, 01001 Vitoria-Gasteiz, Spain. Mycology Department, Fac. of Science, University of the Republic, Julio H. Reissig 565, 11300,(More)
This article represents an updated review of ciliate metallothioneins (Tetrahymena species) including a comparative analysis with regard to well-known metallothioneins (MTs) from other organisms and discussion of their exclusive features. It opens with an introduction to ciliates, summarizing the main characteristics of these eukaryotic microorganisms and(More)
We report the cloning and characterization of two new metallothionein (MT) genes (TrosMTT1 and TrosMTT2), isolated as cDNAs, from the ciliated protozoa Tetrahymena rostrata. The TrosMTT1 inferred protein has been identified as a CdMT and included into the 7a subfamily of Tetrahymena MTs, while TrosMTT2 has been identified as a CuMT (including it into 7b(More)
We studied the cytotoxic effect of the heavy metals Cd, Zn and Cu on three different species of ciliated protozoa isolated from an urban wastewater treatment plant. The order of toxicity was Cd>Cu>>Zn or Cu>Cd>>Zn, depending on the microbial species. In bimetallic (Cd+Zn) treatments, results indicated that, in general, the presence of Zn in the same medium(More)
Two glycoproteins bands isolated from the cyst wall protein pattern of two colpodid ciliates, Colpoda inflata (gp46CI) and Colpoda cucullus (gp46CC) were analysed for their amino acid composition. Both glycoproteins are very rich in glycine and have a relatively high hydrophobicity, containing additionally many leucine and alanine residues. Their high(More)