Ana-Maria Sevcenco

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The hyperthermophilic archaeon Pyrococcus furiosus expresses five aldehyde oxidoreductase (AOR) enzymes, all containing a tungsto-bispterin cofactor. The growth of this organism is fully dependent on the presence of tungsten in the growth medium. Previous studies have suggested that molybdenum is not incorporated in the active site of these enzymes.(More)
The tungsten metallome of the hyperthermophilic archaeon Pyrococcus furiosus has been investigated using electroanalytical metal analysis and native-native 2D-PAGE with the radioactive tungsten isotope (187)W (t(1/2) = 23.9 h). P. furiosus cells have an intracellular tungsten concentration of 29 μM, of which ca. 30% appears to be free tungsten, probably in(More)
Two types of liposomes, commonly used in drug delivery studies, and E. coli bacteria, all prepared in H2O, were resuspended in D2O and measured with Small Angle SpinEcho Neutron Scattering (SESANS). Modeling was performed using correlation functions for solid spheres and hollow spheres. The signal strength and curve shape were more indicative of hollow(More)
The microbial metalloproteome has been largely unexplored. Using the metalloproteomics approach MIRAGE (Metal Isotope native RadioAutography in Gel Electrophoresis) we have been able to explore the soluble Fe and Zn metalloproteome of Escherichia coli. The protein identification by MS/MS typically resulted in several overlapping proteins for each metal(More)
Metalloproteomics is a rapidly developing field of science that involves the comprehensive analysis of all metal-containing or metal-binding proteins in a biological sample. The purpose of this review is to offer an overview of the research involving Metal Isotope native RadioAutography in Gel Electrophoresis (MIRAGE), a powerful new method to visualize and(More)
A combination of techniques to separate and quantify the native proteins associated with a particular transition metal ion from a cellular system has been developed. The procedure involves four steps: (1) labeling of the target proteins with a suitable short-lived radioisotope (suitable isotopes are 64Cu, 67Cu, 187W, 99Mo, 69Zn, 56Mn, 65Ni); (2) separation(More)
Oxo-anion binding properties of the thermostable enzyme ferritin from Pyrococcus furiosus were characterized with radiography. Radioisotopes (32)P and (76)As present as oxoanions were used to measure the extent and the rate of their absorption by the ferritin. Thermostable ferritin proved to be an excellent system for rapid phosphate and arsenate removal(More)
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