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Propionibacterium freudenreichii subsp. shermanii naturally forms propionic acid as the main fermentation product with acetate and succinate as two major by-products. In this study, overexpressing the native propionyl-CoA:succinate CoA transferase (CoAT) in P. shermanii was investigated to evaluate its effects on propionic acid fermentation with glucose,(More)
The effects of CO2 on propionic acid production and cell growth in glycerol or glucose fermentation were investigated in this study. In glycerol fermentation, the volumetric productivity of propionic acid with CO2 supplementation reached 2.94g/L/day, compared to 1.56g/L/day without CO2. The cell growth using glycerol was also significantly enhanced with(More)
Propionibacterium acidipropionici, a Gram-positive, anaerobic bacterium, has been the most used species for propionic acid production from sugars. In this study, the metabolically engineered mutant ACK-Tet, which has its acetate kinase gene knocked out from the chromosome, was immobilized and adapted in a fibrous bed bioreactor (FBB) to increase its acid(More)
Heralded single photons are prepared at a rate of approximately 100 kHz via conditional measurements on polarization-nondegenerate biphotons produced in a periodically poled potassium-titanyl phosphate crystal. The single-photon Fock state is characterized using high-frequency pulsed optical homodyne tomography with a fidelity of (57.6+/-0.1)%. The state(More)
Ipomoea trifida (H.B.K.) G. Don. is the closest wild relative of cultivated sweet potato (I. batatas). The diploid I. trifida is important for sweet potato breeding and construction of transgenic systems. It can also be used to discover functional genes, particularly stress tolerance genes which had been lost during the domestication of sweet potato.(More)
Interspecific hybridization is an important approach to broaden the genetic base and create novel plant forms in breeding programs. However, interspecific hybridization in Ipomoea is very difficult due to the cross incompatibility. Here we report two novel interspecific F1 hybrids between I. batatas (L.) Lam. (2n = 6x = 90) and two wild species, I.(More)
We designed novel quantitative real-time polymerase chain reaction (qPCR) primers for the polyphosphate kinase 1 (ppk1) gene, targeting eight individual "Candidatus Accumulibacter" (referred to as Accumulibacter) clades. An evaluation of primer sets was conducted regarding the coverage, specificity, and PCR efficiency. (i) All primer sets were designed to(More)
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