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PURPOSE To develop a senescence-associated beta-galactosidase histochemistry and bleaching protocol for the primate posterior pole. METHODS Rhesus monkey eyes of different ages were enucleated… (More)
PURPOSE To investigate the relation of senescence-related beta-galactosidase activity and telomere shortening to replicative senescence in cultured human retinal pigment epithelial (RPE) cells. … (More)
METHODS. A human RPI: cell line was serially passaged until 80% of cells were nondividing in a 72-hour 5-bromo-2'-deoxyuridine (BrclU) labeling study. Earlyand late-passage cells were doublestained… (More)