Learn More
The capability to spatially control stem cell orientation and differentiation simultaneously using a combination of geometric cues that mimic structural aspects of native extracellular matrix (ECM) and biochemical cues such as ECM-bound growth factors (GFs) is important for understanding the organization and function of musculoskeletal tissues. Herein,(More)
Investigating the mechanistic influence of the tumor microenvironment on cancer cell migration and membrane blebbing is crucial in the understanding and eventual arrest of cancer metastasis. In this study, we investigate the effect of suspended and aligned nanofibers on the glioma cytoskeleton, cell shape, migration and plasma membrane blebbing dynamics(More)
This paper proposes a method for fabricating suspended micro/nanoscale polymer fibers continuously, in which polymeric micro/nanofibers are formed by drawing and solidification of a viscous liquid polymer solution which is pumped through a glass micropipette. By controlling the drawing parameters, this method is demonstrated to form networks of suspended(More)
Neural Stem Cells (NSCs) are multipotent precursors inhabiting the subventricular and hippocampal subgranular regions of the adult mammalian brain, able to self-renew and differentiate into neurons, astrocytes, and oligodendrocytes, the three primary neural cell types of the adult brain. NSC fate is influenced by the physical and chemical microenvironment(More)
— This paper proposes a nanorobotic fiber fabrication method which uses proximal probes to draw polymer fibers down to few hundred nanometers in diameter and several hundred micrometers in length. Using proximal probes such as Atomic Force Microscope (AFM) and Scanning Tunneling Microscope (STM) or glass micropippettes, liquid polymers dissolved in a(More)
In the body, cells dynamically respond to chemical and mechanical cues from the extracellular matrix (ECM), yet precise mechanisms by which biophysical parameters (stiffness, topography and alignment) affect cell behavior remain unclear. Here, highly aligned and suspended multilayer polystyrene (PS) nanofiber scaffolds are used to study biophysical(More)
Polymeric nanofibers are finding increasing number of applications and hold the potential to revolutionize diverse fields such as tissue engineering, smart textiles, sensors, and actuators. Aligning and producing high aspect ratio fiber arrays (length/diameter > 2 000) in the sub-micron and nanoscale diameters has been challenging due to fragility of(More)
Besides imaging and characterization, proximal probes are proposed to be use as a three-dimensional (3D) nanoscale manipulation and manufacturing tool In this work. We propose 3D nanoscale pulling of liquid polymer micro/nanofibers by precise positioning of atomic force microscope (AFM) nanoprobes and control of polymer solidification. An AFM probe is used(More)
— In-addition to the applications of imaging and characterization, proximal probes are proposed to be used as three-dimensional (3-D) nano-scale manufacturing tools in this paper. Commercially available Atomic Force Microscope (AFM) systems are mainly limited to 1-D or 2-D manipulation tasks, and advanced 3-D nano-manufacturing applications are not(More)