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The capability to spatially control stem cell orientation and differentiation simultaneously using a combination of geometric cues that mimic structural aspects of native extracellular matrix (ECM) and biochemical cues such as ECM-bound growth factors (GFs) is important for understanding the organization and function of musculoskeletal tissues. Herein,(More)
This paper proposes a method for fabricating suspended micro/nanoscale polymer fibers continuously, in which polymeric micro/nanofibers are formed by drawing and solidification of a viscous liquid polymer solution which is pumped through a glass micropipette. By controlling the drawing parameters, this method is demonstrated to form networks of suspended(More)
— This paper proposes a nanorobotic fiber fabrication method which uses proximal probes to draw polymer fibers down to few hundred nanometers in diameter and several hundred micrometers in length. Using proximal probes such as Atomic Force Microscope (AFM) and Scanning Tunneling Microscope (STM) or glass micropippettes, liquid polymers dissolved in a(More)
Besides imaging and characterization, proximal probes are proposed to be use as a three-dimensional (3D) nanoscale manipulation and manufacturing tool In this work. We propose 3D nanoscale pulling of liquid polymer micro/nanofibers by precise positioning of atomic force microscope (AFM) nanoprobes and control of polymer solidification. An AFM probe is used(More)
— In-addition to the applications of imaging and characterization, proximal probes are proposed to be used as three-dimensional (3-D) nano-scale manufacturing tools in this paper. Commercially available Atomic Force Microscope (AFM) systems are mainly limited to 1-D or 2-D manipulation tasks, and advanced 3-D nano-manufacturing applications are not(More)
Neural Stem Cells (NSCs) are multipotent precursors inhabiting the subventricular and hippocampal subgranular regions of the adult mammalian brain, able to self-renew and differentiate into neurons, astrocytes, and oligodendrocytes, the three primary neural cell types of the adult brain. NSC fate is influenced by the physical and chemical microenvironment(More)
Tendon is commonly injured, heals slowly and poorly, and often suffers re-injury after healing. This is due to failure of tenocytes to effectively remodel tendon after injury to recapitulate normal architecture, resulting in poor mechanical properties. One strategy for improving the outcome is to use nanofiber scaffolds and mesenchymal stem cells (MSCs) to(More)
Positioning of the cell division plane is critically important for tissue morphogenesis and architecture. 1 it is therefore not surprising that mitotic spindle orientation must be tightly regulated in living tissues, a phenomenon that is also observed in cells cultured in vitro. Because of the amenability of cultured cells to molecular and physical(More)
A microrobotic method for fabricating multilayered poly(lactic acid) (PLA) biological scaffolds using micropipettes for tissue engineering applications is presented. Biological scaffolds are fabricated over several different substrates by drawing and solidification of a viscous liquid polymer solution pumped continuously through a glass micropipette. The(More)
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