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The two major limitations of Golgi-Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi-Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple,(More)
OBJECTIVES This study was carried out to investigate the effect of rapid eye movement sleep (REMS) deprivation (REMSD) on the cytomorphology of the dorsal raphe (DR) neurons and to evaluate the possible role of REMSD-induced increased noradrenalin (NA) in mediating such effects. METHODS Rats were REMS deprived by the flowerpot method; free moving normal(More)
Golgi-Cox staining has been extensively used in neuroanatomical studies. Despite its unique property to impregnate and show neuronal and glial morphology, its capricious nature, less consistency and long staining time constrain users to use it as first choice. Here we report a modification of the Golgi-Cox method to reliably and differentially stain neurons(More)
Microgravity and sleep loss lead to cognitive and learning deficits. These behavioral alterations are likely to be associated with cytomorphological changes and loss of neurons. To understand the phenomenon, we exposed rats (225-275 g) to 14 days simulated microgravity (SMg) and compared its effects on CA1 hippocampal neuronal plasticity, with that of(More)
An efficient pseudo-spectral numerical method is introduced for calculating a self-consistent field (SCF) approximation for the linear susceptibility of ordered phases in block copolymer melts (sometimes referred to as the random phase approximation). Our method is significantly more efficient than that used in the first calculations of this quantity by(More)
Expression of β1,6-branched N-linked oligosaccharides have a definite association with invasion and metastasis of cancer cells. However, the mechanism by which these oligosaccharides regulate these processes is not well understood. Invasive variants of B16 murine melanoma, B16F10 (parent) and B16BL6 (highly invasive variant) cell lines have been used for(More)
Endophytic fungi isolated from Rauvolfia serpentina, a well known Indian medicinal plant, is used in Ayurveda for treatment of many diseases. Isolated endophytes were screened for their antibacterial activity against pathogenic bacteria. Twenty fungal isolates were recovered from different parts of the host plant and they were characterized for their(More)
Matrix remodeling and invasion of basement membrane are the major determinants of malignant progression. Matrix degrading enzymes play a pivotal role in this process and have been shown to be regulated at multiple levels. Using high metastatic B16F10 and its invasive variant B16BL6 cells, we previously demonstrated that the expression of β1,6 branched(More)
Endophytic fungi were isolated from Gymnema sylvestre, a known medicinal plant for hypoglycemic activity. Isolated endophytes were screened for their antibacterial activity against an array of pathogenic bacteria. The systemic study was made on endophytic fungi of medicinal plant, G. sylvestre growing in different natural habitats of India. A total of 25(More)
" Ship is the significant property of modern society. It's transport Expensive goods and contributes in country security. It's facing many critical emergency cases in sea, that time it's need a adviser, whose having experience to handle the situation. Case-based reasoning (CBR) uses the same technique in solving tasks that needs reference from variety of(More)