Learn More
Coordination between cell populations via prevailing metabolic cues has been noted as a promising approach to connect synthetic devices and drive phenotypic or product outcomes. However, there has been little progress in developing 'controller cells' to modulate metabolic cues and guide these systems. In this work, we developed 'controller cells' that(More)
Improving users' awareness of the imperfections in spatial data has been a research issue explored within Geographic Information Sciences (GIS) for more than 30 years. However, little practical progress has been made toward this objective. Currently, most spatial data producers document information about spatial data quality as part of metadata. However,(More)
UNLABELLED There have been many studies on the relationship between nonpathogenic bacteria and human epithelial cells; however, the bidirectional effects of the secretomes (secreted substances in which there is no direct bacterium-cell contact) have yet to be fully investigated. In this study, we use a transwell model to explore the transcriptomic effects(More)
Escherichia coli were genetically modified to enable programmed motility, sensing, and actuation based on the density of features on nearby surfaces. Then, based on calculated feature density, these cells expressed marker proteins to indicate phenotypic response. Specifically, site-specific synthesis of bacterial quorum sensing autoinducer-2 (AI-2) is used(More)
The exposure and toxicological data used in human health risk assessment are obtained from diverse and heterogeneous sources. Complex mixtures found on contaminated sites can pose a significant challenge to effectively assess the toxicity potential of the combined chemical exposure and to manage the associated risks. A data fusion framework has been(More)
Many reports have elucidated the mechanisms and consequences of bacterial quorum sensing (QS), a molecular communication system by which bacterial cells enumerate their cell density and organize collective behavior. In few cases, however, the numbers of bacteria exhibiting this collective behavior have been reported, either as a number concentration or a(More)
We describe an innovation in the immobilization, culture, and imaging of cells in calcium alginate within microfluidic devices. This technique allows unprecedented optical access to the entirety of the calcium alginate hydrogel, enabling observation of growth and behavior in a chemical and mechanical environment favored by many kinds of cells.
Despite the fact that all geospatial data are, at different levels, imperfect, GIS technologies do not offer much support to GIS users to make them aware of the imperfections and potential limitations in the use of these data. Recent research progresses have been made towards the design of GIS that would take into account data quality. These approaches(More)
While inducing agents are often used to redirect resources from growth and proliferation toward product outputs, they can be prohibitively expensive on the industrial scale. Previously, we developed an autonomously guided protein production system based on the rewiring of E. coli's native quorum sensing (QS) signal transduction cascade. Self-secreted(More)