Amelia L Baldo

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Acylation-stimulating protein (ASP) is the most potent recognized stimulant of triacylglycerol synthesis in human adipocytes. However, its mechanism(s) of action have not yet been elucidated in detail. The present study examines the effects of ASP on membrane transport of glucose and fatty acids in cultured human skin fibroblasts. The data demonstrate that(More)
We have previously characterized an activity from human plasma that markedly stimulates triglyceride synthesis in cultured human skin fibroblasts and human adipocytes. Based on its in vitro activity we named the active component acylation stimulating protein (ASP). The molecular identity of the active serum component has now been determined. NH2-terminal(More)
Through their capacity to store fatty acids as triacylglycerol molecules, adipocytes serve a vital physiologic role. This study presents further evidence that this process can be modulated in human adipocytes by the adipsin/acylation stimulating protein (ASP) pathway and suggests a novel function for the product of this system--ASP. The data demonstrate the(More)
Acylation Stimulating Protein (ASP) is a small (mol wt 14,000), basic (pI 9.0) protein present in human plasma. When examined in vitro with normal human cultured skin fibroblasts and adipocytes, ASP appears to be the most potent stimulant of triglyceride synthesis yet described. In this study, a competitive ELISA assay for ASP has been developed using(More)
Acylation Stimulating Protein (ASP) was recently purified to homogeneity from human plasma and shown to be identical to C3adesArg. ASP stimulates triglycerides synthesis in human skin fibroblasts and primary human adipocytes. In vitro differentiation of human preadipocytes to mature fat cells results in increased expression and accumulation of ASP in the(More)
An HPLC method was developed for the quantitative determination of 2,4,6-triiodoresorcinol (I3R), 2-(2',4'-dihydroxy-3',5'-diiodobenzoyl)benzoic acid, resorcinol, phthalic acid, and sodium iodide in the colour additive FD&C Red No. 3 (erythrosine) (R3). Due to the fast decomposition of I3R in aqueous solutions, the dye portions analysed were dissolved in(More)
Gemfibrozil is an effective hypotriglyceridemic agent. Its mechanism of action has not been determined with certainty, but it is generally thought to act by virtue of an effect on lipoprotein lipase. However, this study was designed to test the hypothesis that gemfibrozil directly affects intracellular triglyceride synthesis in peripheral cells, and indeed,(More)
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