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Nineteen cashew accessions were analysed with 50 random primers, 12 ISSR primers and 6 AFLP primer pairs to compare the efficiency and utility of these techniques for detecting variation in cashew germplasm. Each marker system could discriminate between all of the accessions, albeit with varied efficiency of polymorphism detection. AFLP exhibited maximum(More)
Indian cashew breeding programme has produced 24 selections and 11 hybrids with increased yield and excellent nut characters. Molecular profiles of these varieties were developed using a combination of five RAPD and four ISSR primers pre-selected for maximum discrimination and repeatability. A total of 94 markers were generated which discriminated all the(More)
Microsatellite or simple sequence repeat (SSR) markers are the preferred markers for genetic analyses of crop plants. The availability of a limited number of such markers in bitter gourd (Momordica charantia L.) necessitates the development and characterization of more SSR markers. These were developed from genomic libraries enriched for three dinucleotide,(More)
We report the development of a microarray platform for rapid and cost-effective genetic mapping, and its evaluation using rice as a model. In contrast to methods employing whole-genome tiling microarrays for genotyping, our method is based on low-cost spotted microarray production, focusing only on known polymorphic features. We have produced a genotyping(More)
Cashew (Anacardium occidentale L.), introduced into India about 400 years ago, is distributed widely in the coastal regions as spontaneous populations as well as in cultivation. Despite the plant's commercial exploitation, little is known about its actual introduction and diversification. We attempted to construct these events by investigating the level of(More)
Molecular characterization of 19 advanced cultivars and landraces of brinjal was carried out using RAPD and ISSR markers. Twenty-nine RAPD primers generated a total of 240 amplified fragments, while 23 anchored and non-anchored ISSR primers produced 299 fragments. Of these, 66 (27.5%) RAPD and 56 (18.73%) ISSR fragments were polymorphic. All the cultivars(More)
The preparation method of metal based Indian traditional drugs involves conversion of a pure metal into its oxide by repeated high temperature calcination cycles. In this work, the effect of number of calcination cycles followed in the preparation of tin oxide based Ayurvedic drug, 'vanga bhasma' was studied by a systematic characterization of the drug(More)
Twelve Indian mango cultivars were fingerprinted using anchored-ISSR primers. Out of total 161 bands amplified, 113 (70.2%) were polymorphic, the polymorphism ranging from 50% to 94.1% depending upon the primer. One primer [5′ HVH(CA)7T 3′] with highest genotype index could uniquely identify each of the cultivars studied. Fingerprints based on polymorphic(More)
Ambika B Gaikwad ambikanbpgr.ernet.in. The quality of DNA, obtained by various protocols and subjected to. rapd pcr protocol pdf RAPD is a PCR based technique which involves the use of single arbitrary. Advantages disadvantages, limitations and applications of RAPD.RAPD is a modification of the PCR in which a single, short and arbitrary. Therefore, RAPD(More)
— The low carbon steel (LCS) electrodes were polarized under cyclic voltammetric conditions in sodium oxalate solution at room temperature in order to understand the different processes that occur at the electrode surface. This study suggests that, a continuous dissolution of the LCS electrode occurs in oxalate solution. In order to understand the observed(More)