Amber Gustafson

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A modified one-dimensional thin-layer chromatographic procedure is presented for the separation from tissues of five phospholipids (phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine and sphingomyelin) and three lysophospholipids (lysophosphatidylserine, lysophosphatidylethanolamine and lysophosphatidylcholine). This is(More)
Lipid extraction efficiency is variable when different methods are used on the same tissue. After a systematic comparison on erythrocytes, we found that this variability was due to several factors, such as the addition of alcohol and chloroform as a mixture, the use of repeated extractions or partition procedures after the extraction. Most important,(More)
The effects of a 500 ml 4 hr infusion of 10% fat emulsion (Lipovenös) on the lipid content of plasma lipoproteins and blood cells, and on the platelet aggregation rate, were studied in ten volunteers. The infusion resulted in a significant (P < 0.05) initial increase in plasma total triglycerides (more than 5 times), phospholipids (28.7%) and free(More)
Reasons for the variation in erythrocyte lipid extraction were investigated. We found that there was an uneven distribution of lipids after filtration (up to 10% accumulated in the filtrates) or centrifugation (up to 10% in the top fraction) of several apparently monophasic extraction systems. The uneven distribution was more pronounced in the systems(More)
Several methods for ganglioside extraction from erythrocytes have been compared. Our results show that ganglioside extraction is unfavourably affected by the addition of the solvents as a mixture and by the use of less polar solvents and by a lower total solvent-to-sample ratio. In accordance with our previous observation on cholesterol and phospholipids,(More)
Synthesis of bile salts is regulated through negative feedback inhibition by bile salts returning to the liver. Individual bile salts have not been distinguished with regard to inhibitory potential. We assessed inhibition of bile salt synthesis by either cholate or its taurine conjugate in bile fistula rats. After allowing synthesis to maximize, baseline(More)
D-myo-inositol-1,2,6 trisphosphate (PP56) was infused in rats for 3 hr at a rate of 30.28 microM/hr/kg body weight. The cholesterol content in plasma, erythrocytes, the aorta, and the liver was monitored before and at the end of the infusion and at 1 and 21 hr after the infusion. We found that the aortic total and free cholesterol content was reduced after(More)
In the assay for total cholesterol in lipid extracts by enzymatic methods, it was found necessary to redissolve the lipid prior to the reagent addition. Isopropyl alcohol was found to be best at promoting the colorimetric reaction. As little as 1.45 micrograms free cholesterol and 1.8 micrograms esterified cholesterol could be determined after 15 min(More)
Cultured Babesia bovis and Babesia bigemina were recovered from liquid nitrogen storage nearly 30 years after they were cryopreserved. Four cattle were compared as donors of erythrocytes and serum for microaerophilous stationary phase (MASP) cultures for recovery of B. bigemina. Erythrocytes and serum from only one (#913) of the four animals supported(More)
The effect on cholesterol transport of an intravenous infusion of a fat emulsion (10%) Intralipid or 10% Lipovenös) in vivo was investigated in the rat. Intralipid (1.85 ml/hr/kg body weight in rts for 3 hr) caused a reduction (P < 0.05) in free cholesterol in the aorta (by 25%), in plasma high-density lipoproteins (64%) and in erythrocytes (11%) with a(More)