Amaya Azqueta

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The essential comet assay: a comprehensive guide to measuring DNA damage and repair

Amaya Azqueta, Andrew R Collins
Archives of Toxicology
2013

The comet assay (single cell gel electrophoresis) is the most common method for measuring DNA damage in eukaryotic cells or disaggregated tissues. The assay depends on the relaxation of supercoiled… (More)

DNA oxidation: investigating its key role in environmental mutagenesis with the comet assay.

Amaya Azqueta, Sergey S. Shaposhnikov, Andrew R Collins
Mutation research
2009

DNA oxidation, which can have potentially serious mutagenic consequences, commonly accompanies exposure to environmental mutagens. Oxidised bases can be measured chromatographically, but spurious… (More)

Variation in the measurement of DNA damage by comet assay measured by the ECVAG inter-laboratory validation trial.

Lykke Forchhammer, Clara Johansson, +22 authors Peter Møller
Mutagenesis
2010

The comet assay has become a popular method for the assessment of DNA damage in biomonitoring studies and genetic toxicology. However, few studies have addressed the issue of the noted… (More)

Towards a more reliable comet assay: optimising agarose concentration, unwinding time and electrophoresis conditions.

Amaya Azqueta, Kristine Bjerve Gutzkow, Gunnar Brunborg, Andrew R Collins
Mutation research
2011

The comet assay is now the method of choice for measuring most kinds of DNA damage in cells. However, due to the lack of a standardised protocol inter-laboratory comparisons are of limited value. The… (More)

DNA repair as a biomarker in human biomonitoring studies; further applications of the comet assay.

Andrew R Collins, Amaya Azqueta
Mutation research
2012

DNA repair plays a major role in maintaining genetic stability, and so measurement of individual DNA repair capacity should be a valued tool in molecular epidemiology studies. The comet assay (single… (More)

Effect of processed and red meat on endogenous nitrosation and DNA damage

Annemiek M.C.P. Joosen, Gunter G C Kuhnle, +5 authors Sheila Anne Bingham
Carcinogenesis
2009

Haem in red meat (RM) stimulates the endogenous production of mutagenic nitroso compounds (NOC). Processed (nitrite-preserved red) meat additionally contains high concentrations of preformed NOC. In… (More)

Comet assay to measure DNA repair: approach and applications

Amaya Azqueta, Jana Slyskova, Sabine A. S. Langie, Isabel O’Neill Gaivão, Andrew R Collins
Front. Genet.
2014

Cellular repair enzymes remove virtually all DNA damage before it is fixed; repair therefore plays a crucial role in preventing cancer. Repair studied at the level of transcription correlates poorly… (More)

A comparative performance test of standard, medium- and high-throughput comet assays.

Amaya Azqueta, Kristine Bjerve Gutzkow, +4 authors Andrew R Collins
Toxicology in vitro : an international journal…
2013

A serious limitation of the conventional comet assay (single cell gel electrophoresis) is the restriction on the number of samples that can be processed in one experiment, imposed by the size of the… (More)

Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead

William H. Goodson, Leroy Lowe, +170 authors Zhiwei Hu
Carcinogenesis
2015

Lifestyle factors are responsible for a considerable portion of cancer incidence worldwide, but credible estimates from the World Health Organization and the International Agency for Research on… (More)

Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation.

Sergey A Shaposhnikov, Amaya Azqueta, +7 authors Andrew R Collins
Toxicology letters
2010

The comet assay is widely used to measure DNA damage and repair in basic research, genotoxicity testing and human biomonitoring. The conventional format has 1 or 2 gels on a microscope slide, 1… (More)

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