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Defective DNA repair by homologous recombination (HR) is thought to be a major contributor to tumorigenesis in individuals carrying Brca1 mutations. Here, we show that DNA breaks in Brca1-deficient cells are aberrantly joined into complex chromosome rearrangements by a process dependent on the nonhomologous end-joining (NHEJ) factors 53BP1 and DNA ligase 4.(More)
Dose response and time course of the effects of TGF-on RNA levels of various genes. Dose response. MMC were treated with 0, 2, 5 or 10 ng/ml of TGF- for 24 hours. Expression levels of miR-192 (a), RP23 (b), miR-216a (c), miR-217 (d), Zeb1 (e), Zeb2 (f), Col1a2 (g) and Pten (h) were examined by RT-qPCR (mean and s.e.m., n=3). Time course. MMC were treated(More)
To characterize the repair pathways of chromosome double-strand breaks (DSBs), one approach involves monitoring the repair of site-specific DSBs generated by rare-cutting endonucleases, such as I-SceI. Using this method, we first describe the roles of Ercc1, Msh2, Nbs1, Xrcc4, and Brca1 in a set of distinct repair events. Subsequently, we considered that(More)
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