Promises and Challenges of the Type Three Secretion System Injectisome as an Antivirulence Target.
- Alyssa C. Fasciano, L. Shaban, J. Mecsas
- BiologyEcoSal Plus
- 1 February 2019
The T3SSi has been an attractive target for identifying small molecules, peptides, and monoclonal antibodies that inhibit its function to render the pathogen avirulent, and research into these antivirulence therapies requires combined efforts from both pharmaceutical companies and academic labs.
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers.
- Alyssa C. Fasciano, S. Blutt, M. Estes, J. Mecsas
- Biology, MedicineJournal of Visualized Experiments
- 26 July 2019
The following protocol describes a method for the differentiation of M cells in a transwell epithelial polarized monolayer system of the intestine using human ileal enteroids, which can be applied to the study of M cell development and function.
Yersinia pseudotuberculosis YopE prevents uptake by M cells and instigates M cell extrusion in human ileal enteroid-derived monolayers
- Alyssa C. Fasciano, Gayani Dasanayake, J. Mecsas
- BiologybioRxiv
- 1 January 2021
Human ileal enteroid-derived monolayers containing five intestinal cell types including M cells are used to study the interactions between the enteric pathogen, Yersinia pseudotuberculosis (Yptb) and M cells, finding that Yops impede M cell function, indicating that early infectious stage Yptb more effectively penetrates M cells while the host may defend against M cell penetration of host-adapted YptB.
New Age Strategies To Reconstruct Mucosal Tissue Colonization and Growth in Cell Culture Systems.
- Alyssa C. Fasciano, J. Mecsas, R. Isberg
- BiologyMicrobiology spectrum
- 8 March 2019
The latest studies using organoids and engineering technology that have been developed and applied to the study of host-pathogen interactions in mucosal tissues are discussed.
Promises and Challenges of the Type Three Secretion System-Injectisome as an Anti-Virulence Target
- Alyssa C. Fasciano, L. Shaban, J. Mecsas
- Biology
- 2019
The T3SSi has been an attractive target for identifying small molecules, peptides, and monoclonal antibodies that inhibit its function to render the pathogen avirulent, and research into these anti-virulence therapies requires combined efforts from both pharmaceutical companies and academic labs.
Eat your Vitamin A: A role for Retinoic Acid in the Development of M cells.
- Alyssa C. Fasciano, J. Mecsas
- Medicine, BiologyGastroenterology
- 12 May 2020
Detection of Cells Translocated with Yersinia Yops in Infected Tissues Using β-Lactamase Fusions.
- G. T. Nguyen, Anne L. McCabe, Alyssa C. Fasciano, J. Mecsas
- BiologyMethods in molecular biology
- 2019
How to generate Yop-TEM constructs, and how to detect, quantify, isolate, and study YoTEM containing cells in murine tissues during infection and in ex vivo tissues by cell sorting and flow cytometry analysis are described.
Promises and Challenges of the Type Three Secretion System Injectisome as an Antivirulence Target
- Alyssa C. Fasciano, L. Shaban, J. Mecsas
- BiologyProtein Secretion in Bacteria
- 12 September 2019