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Angiotensin II (Ang II) inhibits the cardiac sarcolemmal Na(+)-K(+) pump via protein kinase (PK)C-dependent activation of NADPH oxidase. We examined whether this is mediated by oxidative modification of the pump subunits. We detected glutathionylation of beta(1), but not alpha(1), subunits in rabbit ventricular myocytes at baseline. beta(1) Subunit(More)
Distillers grains with solubles (DGS) is the major coproduct of ethanol production, usually made from corn, which is fed to dairy cattle. It is a good protein (crude protein, CP) source (>30% CP) high in ruminally undegradable protein (approximately 55% of CP) and is a good energy source (net energy for lactation of approximately 2.25 Mcal/kg of dry(More)
BACKGROUND Glutathionylation of endothelial nitric oxide synthase (eNOS) "uncouples" the enzyme, switching its function from nitric oxide (NO) to O2(•-) generation. We examined whether this reversible redox modification plays a role in angiotensin II (Ang II)-induced endothelial dysfunction. METHODS AND RESULTS Ang II increased eNOS glutathionylation in(More)
Defining the gonadal effects of FSH distinct from those of LH remains difficult. We have characterized and compared the level of Sertoli and germ cell development in three mouse models recently created to isolate FSH activity from LH effects. Two models used LH-deficient hypogonadal (hpg) mice to selectively study either pituitary-independent transgenic(More)
Whole-cell patch-clamp measurements of the current, Ip, produced by the Na(+),K(+)-ATPase across the plasma membrane of rabbit cardiac myocytes show an increase in Ip over the extracellular Na(+) concentration range 0-50 mM. This is not predicted by the classical Albers-Post scheme of the Na(+),K(+)-ATPase mechanism, where extracellular Na(+) should act as(More)
BACKGROUND inhibition of L-type Ca(2+) current contributes to negative inotropy of β(3) adrenergic receptor (β(3) AR) activation, but effects on other determinants of excitation-contraction coupling are not known. Of these, the Na(+)-K(+) pump is of particular interest because of adverse effects attributed to high cardiac myocyte Na(+) levels and(More)
This paper describes a real-time approach for foreground segmentation in image sequences taken from a stationary camera, as a first preprocessing step for object extraction. The proposed method combines a background subtraction method with the extensible inclusion of context information. It currently considers bidirectional temporal change detection as the(More)
The determination of the characteristic frequencies of an electromechanical resonance does not provide enough data to obtain the material properties of piezoceramics, including all losses, from complex impedance measurements. Values of impedance around resonance and antiresonance frequencies are also required to calculate the material losses. Uncoupled(More)