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Regeneration of flax (Linum usitatissimum) following transformation by either Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector, or Agrobacterium rhizogenes carrying an unmodified Ri plasmid, was examined. Hypocotyl and cotyledon explants inoculated with A. tumefaciens formed transformed callus, but did not regenerate transformed shoots either(More)
The oncogenic plasmids of Agrobacterium, the Ti-plasmids, carry genes that enable their bacterial host to catabolize opines. Opines are unusual amino acid derivatives that are only produced in crown gall tumours incited by oncogenic strains of Agrobacterium. The 2 opines, octopine and nopaline, are degraded by Agrobacterium strains carrying the octopine or(More)
The conjugative behaviour of nopaline and agropine Ti-plasmids has been investigated. Using a technique which avoids enrichment of transconjugants on a mating medium we have shown that preculture in the presence of agrocinopines A or B of donor strains harbouring nopaline Ti plasmids promotes plasmid transfer whereas preculture of the same strains in the(More)
Root induction on flax (Linum usitatissimum L.) cotyledon explants by Agrobacterium rhizogenes strain 1855 is markedly increased by co-inoculation with disarmed A. tumefaciens strain LBA 4404 containing a plasmid carrying the tzs gene of pTiC58. Most of the roots (estimated to be more than 90%) were transformed. This effect is most likely due to the(More)
Agrobacterium radiobacter strain K84 is used commercially for the biological control of crown gall. It contains the conjugative plasmid pAgK84, which encodes the synthesis of agrocin 84, an antibiotic that inhibits many pathogenic agrobacteria. A breakdown of control is threatened by the transfer of pAgK84 to pathogens, which then become resistant to(More)
I have some affinity with Daniel McAlpine. We were both born in Scotland and both came to Australia where we studied plant diseases. He arrived in Melbourne in 1884 and I in Adelaide in 195167 years later. In fact before coming to Australia, I knew of Daniel McAlpine through my teacher and mentor Malcolm Wilson who, like McAlpine, was a rust enthusiast. I(More)
Conjugal transfer of the nopaline Ti-plasmid pTiC58 is inducible by the phosphorylated opines, agrocinopines A and B. Although the uninduced level of transfer is negligible (< 10−7 per donor), some transconjugants have been isolated from crosses performed in the absence of agrocinopine. These transconjugants harbour mutant Ti-plasmids that transfer(More)
Knowledge of the composition of human urinary calculi within a geographical area can lead to an understanding of the epidemiology of renal stone disease. Studies in the Durban area, with its high incidence of kidney and bladder stones, indicate that calcium oxalate is the overwhelmingly dominant (circa 80%) component, followed by uric acid. An important(More)