Alison Agnew

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Beta-barrel transmembrane (bbtm) proteins are a functionally important and diverse group of proteins expressed in the outer membranes of bacteria (both gram negative and acid fast gram positive), mitochondria and chloroplasts. Despite recent publications describing reasonable levels of accuracy for discriminating between bbtm proteins and other proteins,(More)
TMB-Hunt is a program that uses a modified k-nearest neighbour (k-NN) algorithm to classify protein sequences as transmembrane beta-barrel (TMB) or non-TMB on the basis of whole sequence amino acid composition. By including differentially weighted amino acids, evolutionary information and by calibrating the scoring, a discrimination accuracy of 92.5% was(More)
The blood dwelling stages of schistosomes have acetylcholinesterase (AChE) and nicotinic-like acetylcholine receptors (nAChR) on their teguments. Both AChE and nAChR are concentrated on the dorsal surface of the adult male, a major surface for nutrient uptake for the worm pair. Exposure of tegumental AChE and nAChR to acetylcholine (ACh), the natural ligand(More)
Acetylcholinesterase (AChE) on the surface of the parasitic blood fluke Schistosoma is the likely target for schistosomicidal anticholinesterases. Determination of the molecular structure of this drug target is key for the development of improved anticholinesterase drugs and potentially a novel vaccine. We have recently cloned the cDNA encoding the AChE(More)
The host-parasite relationships of two geographical isolates of Schistosoma haematobium in CBA mice are described and compared to previous reports on this parasite in other experimental hosts and in man. The mean percentage establishment of worms in mice was 17% and was not affected by the age or sex of the host. Adult worm burdens remained constant over 20(More)
Blood dwelling stages of schistosomes have acetylcholinesterase (AChE) on their teguments. As an initial step towards understanding the function of tegumental AChE, we have used specific ligand-binding assays to identify nicotinic acetylcholine receptors (nAChR) on the schistosome surface. AChR could not be detected on migratory stages using fluoroscein(More)
Understanding the dynamics of schistosome infections is problematic because direct measurements of worm burden are not possible. Hitherto, the relative intensity of infection has been estimated by the number of parasite eggs excreted. Egg excretion is assumed to have a consistent relationship with worm burden with duration of infection. We have tested this(More)
Mouse infection models are described that demonstrate reduction in the rate of egg production in Schistosoma haematobium worms 6-10 weeks after the onset of oviposition and loss of Schistosoma bovis worms around 10 weeks after infection. Neither phenomenon has been shown in Schistosoma mansoni- or Schistosoma japonicum-infected mice. The immunological basis(More)
The cDNAs for two novel neuronal-type nicotinic acetylcholine receptor (nAChR) subunits have been cloned and characterised from the parasitic trematode blood fluke Schistosoma haematobium. One of these encodes a putative nAChR alpha-subunit named ShAR1alpha, whilst the second encodes a potential non-alpha subunit, ShAR1beta. These ShARs possess the key(More)