Alis Guillén

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Enzymatically dissociated mouse FDB muscle fibers, loaded with Fura-2 AM, were used to study the effect of mitochondrial uncoupling on the capacitative Ca2+ entry, SOCE. Sarcoplasmic reticulum (SR) Ca2+ stores were depleted by repetitive exposures to high K+ or 4-chloro-m-Cresol (4-CmC) in the absence of extracellular Ca2+. SR Ca2+ store replenishment was(More)
We report the use of the fluorescent dye CalciumOrange-5N (CaOr-5N) as a specific mitochondria Ca2+ marker in enzymatically dissociated mouse FBD muscle fibers. Using laser scanning confocal microscopy and the dyes Mitotracker Green (MTG), di-8-ANEPPS and endoplasmic reticulum tracker green (ERTG), we determined the relative position of mitochondria,(More)
Eucalyptus tereticornis is a plant used in traditional medicine to control diabetes, but this effect has not been proved scientifically. Here, we demonstrated through in vitro assays that E. tereticornis extracts increase glucose uptake and inhibit their production in insulin-resistant C2C12 and HepG2 cells, respectively. Furthermore, in a nutritional model(More)
Jatropha gossypifolia L. (Euphorbiaceae) is a plant widely used in the treatment of type 2 diabetes mellitus (T2DM), but there are few scientific reports validating its activity in this area. In this work and through a bioguided assay, a crude extract stimulated glucose uptake in C2C12 myotubes up to 30%, thereby reducing insulin resistance induced by fatty(More)
We have quantified Ca2+ entry through store operated calcium channels in mice muscle fibres, measuring the rates of change of myoplasmic [Ca2+], d[Ca2+]myo/dt, and of Ca2+ removal, d[Ca2+]Removal/dt, turning store operated calcium entry (SOCE) ON, and OFF, by switching on or off external Ca2+. In depleted fibres, poisoned with 10 μM cyclopiazonic acid SOCE(More)
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