Alina V. Martynova

Learn More
Restriction fragment length polymorphism (RFLP) analysis is an economic and fast technique for molecular typing but has the drawback of difficulties in accurately sizing DNA fragments and comparing banding patterns on agarose gels. We aimed to improve RFLP for typing of the important human pathogen Streptococcus pneumoniae and to compare the results with(More)
So far the prevalent method of the identification of pneumococci in the routine diagnostic practice has included three tests: sensitivity to optoxin, solubility in biliary salts and reaction with omniserum. At present optoxin-resistant pneumococcal strains, as well as those giving dual reaction to solubility in biliary salts, occur sufficiently often(More)
The aim of the study was to determine nosological structure, sex and age of patients with non-invasive pneumococcal infections (otitis and sinusitis). Medical cards of 80 inpatients and results of clinico-microbiological tests have been analysed. Otitis and sinusitis morbidity is about 12+/-0.54 and 36+/-1.04 per 10 000, respectively. Acute otitis occurred(More)
Despite modern achievements in diagnostics and treatment, invasive and non-invasive pneumococcal infections remain a topical public health problem. To a large extent, it is connected with the absence or inconsistence of evidence-based information on this kind of infection. In this paper, retrospective analysis of the incidence of pneumococcal infections was(More)
Pneumococcal infection is one of the most important problems of the modern medicine. Need for organization of system of epidemiological surveillance for this infection with obligatory assessment of epidemic genovariants by molecular biological methods is evident. Assessment of pneumococci isolated from patients and carriers by using multilocus sequence(More)
Despite advances in the diagnosis and treatment of pneumococcal infections, effective antibiotic therapy for pneumococcal pneumonias remains to be a topical problem. The investigation was undertaken to study the specific features of development of antibiotic resistance in S. pneumoniae strains isolated in patients with pneumococcal pneumonias. In the(More)
We have developed a simple HPLC method for analysis of the dehydroepiandrosterone sulfate (DHEA-sulfate) in serum with use a new procedure of solid-phase extraction (SPE) on hyper cross-linked polystyrene (Purosep-200) and fast chromatographic separation on the monolithic column under isocratic elution and UV detection at 200 nm. Complete SPE procedure(More)