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Objective and Design: As acute inflammation is known to cause a reduction in hepatic P-Glycoprotein (PGP) expression and activity in rats, we tested the hypothesis that the pro-inflammatory cytokines interleukin (IL-)1β and IL-6 also mediate reductions in PGP.¶Methods: Hepatocytes were incubated with 0-50 ng/ml of cytokine for 24-72 h. PGP/mdr expression(More)
The multifunctional enzyme tissue transglutaminase (TG2) contributes to the development and progression of several cardiovascular diseases. Extracellular rather than intracellular TG2 is enzymatically active, however, the mechanism by which it is exported out of the cell remains unknown. Nitric oxide (NO) is shown to constrain TG2 externalization in(More)
Nitric oxide (NO) can modulate arterial stiffness by regulating both functional and structural changes in the arterial wall. Tissue transglutaminase (TG2) has been shown to contribute to increased central aortic stiffness by catalyzing the cross-linking of matrix proteins. NO S-nitrosylates and constrains TG2 to the cytosolic compartment and thereby holds(More)
Arginase constrains endothelial nitric oxide synthase activity by competing for the common substrate, l-Arginine. We have recently shown that inducible nitric oxide synthase (NOS2) S-nitrosates and activates arginase 1 (Arg1) leading to age-associated vascular dysfunction. Here, we demonstrate that a direct interaction of Arg1 with NOS2 is necessary for its(More)
Neomycin, a selective inhibitor of the phosphoinositide metabolism, depending on its concentration and the incubation time, depressed the K+-contractures and contractions of m. sartorius R. ridibunda caused by a single or a tetanic electrostimulation. In the experiments on m. rectus abdominis, neomycin inhibited the Ach- and oubaine contractures but not the(More)
The glycerol was shown to lead to a considerable though transient increase in the contractile amplitude of the m. sartorius. Prolonged incubation of the muscle in the Ringer and Ca-free solutions in presence of glycerol led to a decrease in the response following potentiation. The potentiation due to glycerol seems to be caused by Ca2+ release which, in its(More)
The potentiation of contractile responses during prolonged incubation of frog sartorius muscles in a conventional Ringer solution and in a calcium-free Ringer solution which contained 400 mM of urea, acetamide or ethylene glycol was observed when stimulation with single electric impulses and with caffeine was carried out. When the muscles were exposed to a(More)