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Accurate, sensitive, multiplexed detection of biomarker proteins holds significant promise for personalized cancer diagnostics. Here we describe the incorporation of a novel on-line chamber to capture cancer biomarker proteins on magnetic beads derivatized with 300,000 enzyme labels and 40,000 antibodies into a modular microfluidic immunoarray. Capture and(More)
Multielectronic O(2) reduction reactions (ORR) at Pt surface (and at Au surface for comparison purpose) were examined both in water and in organic solvents using a strategy based on radical footprinting and scanning electrochemical microscopy (SECM). Experiments reveal a considerable and undocumented production of OH radicals when O(2) is reduced at a Pt(More)
A surface sensitive to reactive oxygen species (ROS) was prepared by reduction of a diazonium salt on glassy carbon electrode followed by the chemical coupling of glutathione (GSH) playing the role of an antioxidant species. The presence of active GSH was characterized through spectroscopic studies and electrochemical analysis after labeling of the -SH(More)
mM thick) was purchased from American Durafilm. Lyophilized 99% bovine serum albumin (BSA), sterile-filtered bovine calf serum, gold (III) chloride trihydrate, 1-dodecane thiol, tetrocylammonium bromide, sodium borohydride, 3-mercaptopropionic acid (MPA), N-hydroxysulfosuccinimide (NHSS), 1-Ethyl-3-[-3-dimethylaminopropyl] carbodimmide hydrochloride (EDC),(More)
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