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Real-time RT-PCR normalisation; strategies and considerations
Real-time RT-PCR has become a common technique, no longer limited to specialist core facilities. It is in many cases the only method for measuring mRNA levels of vivo low copy number targets ofExpand
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The continuing 2019-nCoV epidemic threat of novel coronaviruses to global health — The latest 2019 novel coronavirus outbreak in Wuhan, China
The city of Wuhan in China is the focus of global attention due to an outbreak of a febrile respiratory illness due to a coronavirus 2019-nCoV. In December 2019, there was an outbreak of pneumonia ofExpand
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Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study
Summary Background Middle East respiratory syndrome (MERS) is a new human disease caused by a novel coronavirus (CoV). ClinicalExpand
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Hospital outbreak of Middle East respiratory syndrome coronavirus.
BACKGROUND In September 2012, the World Health Organization reported the first cases of pneumonia caused by the novel Middle East respiratory syndrome coronavirus (MERS-CoV). We describe a cluster ofExpand
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Coronaviruses — drug discovery and therapeutic options
In humans, infections with the human coronavirus (HCoV) strains HCoV-229E, HCoV-OC43, HCoV-NL63 and HCoV-HKU1 usually result in mild, self-limiting upper respiratory tract infections, such as theExpand
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Validation of housekeeping genes for normalizing RNA expression in real-time PCR.
Analysis of RNA expression using techniques like real-time PCR has traditionally used reference or housekeeping genes to control for error between samples. This practice is being questioned as itExpand
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Advances in the development of new tuberculosis drugs and treatment regimens
Despite the introduction 40 years ago of the inexpensive and effective four-drug (isoniazid, rifampicin, pyrazinamide and ethambutol) treatment regimen, tuberculosis (TB) continues to causeExpand
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The WHO 2014 global tuberculosis report--further to go.
This article discusses how the 19th WHO global tuberculosis report 2014 provides an opportunity to think about the global tuberculosis strategy, and to assess just how much further effort is neededExpand
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Best drug treatment for multidrug-resistant and extensively drug-resistant tuberculosis.
Multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis are generally thought to have high mortality rates. However, many cases can be treated with the right combination andExpand
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Epidemiology and clinical management of Legionnaires' disease.
Legionnaires' disease is an important cause of community-acquired and hospital-acquired pneumonia. Although uncommon, Legionnaires' disease continues to cause disease outbreaks of public healthExpand
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