Alicia Gostnell

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Group B Streptococcus detection directly from Copan ESwab collected samples, using the BD Max GBS assay, was evaluated on receipt in the laboratory and after 24 h at room temperature. Results were compared to those using Lim broth enrichment PCR and culture. No significant difference was observed between 24 h ESwab and Lim broth PCRs.
A multiplex real-time PCR was validated on the BD Max open system to detect different Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium spp. directly from clinical samples. The PCR results were compared to those with traditional cultures. The multiplex PCR assay was found to be a specific and sensitive method for the rapid(More)
Gastrointestinal disease accounts for significant morbidity and mortality worldwide and may be caused by a variety of agents, including bacterial, viral, and parasitic pathogens (1, 2, 3). Rapid molecular multiplex testing has recently been introduced into enteric diagnostics and is revolutionizing patient diagnosis and treatment for diarrheal diseases (4,(More)
Staphylococcus aureus is the most common pathogen involved in skin and soft tissue infections, and it is the principal cause of surgical site infections (1–3). Most of the laboratory methods for detecting S. aureus and methicillin-resistant S. aureus (MRSA) from wounds require incubation time and do not support rapid decisions for selection of the most(More)
A new multiplex PCR test was designed to detect Mycobacterium chelonae, Mycobacterium abscessus group, and Mycobacterium fortuitum complex on the BD MAX System. A total of 197 clinical samples previously submitted for mycobacterial culture were tested using the new protocol. Samples were first treated with proteinase K, and then each sample was inoculated(More)
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