Alice Subtelney

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Flow cytometry has been used to detect estrogen receptor (ER) in intact cells, using 1-(N)- fluoresceinyl estrone thiosemicarbazone ( 17FE ), a ligand shown to have sufficient affinity and specificity to identify high-affinity receptors. For each concentration of 17FE , two fluorescent distributions were obtained: (a) "total" fluorescence due to 17FE alone;(More)
Flow cytometry has been used to detect estrogen receptor (ER) ¡nintact cells, using 1-{/v>fluoresceinyl estrone thiosemicarbazone (17FE), a ligand shown to have sufficient affinity and specificity to identify high-affinity receptors. For each concentra tion of 17FE, two fluorescent distributions were obtained: (a) "total" fluorescence due to 17FE alone;(More)
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