Learn More
Systematic examination of photomontages revealed two types of synaptic glomeruli in laminae II-III. Type I glomeruli have a dark small central (C) terminal of indented contour with closely packed spherical vesicles of variable diameter and few mitochondria. Among the peripheral terminals there are dendritic spines and a few presynaptic dendritic spines (V1(More)
On the basis of dendroarchitecture and cell body shape, complemented with morphometry of dendritic ramification, four major neuronal types were distinguished in lamina I of the spinal cord of the rat. (I) Fusiform spiny cells (39% of impregnated neurons) have longitudinal spindle-shaped perikarya with bipolar, less frequently unipolar, dendritic trees rich(More)
A significant fall in the number of GABA-immunoreactive cells in laminae I-III of the rat spinal cord occurred in the somatotopic area of projection of the sciatic nerve after nerve transection. The decrease started at 2 weeks post-neurectomy, and at 4 weeks ipsilateral mean cell numbers were approximately 72% of contralateral control values. Similarly, the(More)
In this study the desensitizing power of increasing concentrations of resiniferatoxin applied topically to the bladder mucosa, and the irritating properties of the most effective desensitizing dose, were determined with the aid of the spinal expression of the proto-oncogene c-fos. Desensitization was assessed by the decrease in the number of(More)
Retrogradely labelled lamina I neurons were studied after intrathalamic injections of free horseradish peroxidase mixed with dimethylsulphoxide, wheat germ agglutinin conjugated with horseradish peroxidase, and subunit B of cholera toxin. The first two tracers revealed only the perikaryal shape and the orientation of primary dendrites, while cholera toxin(More)
The projections of the superficial dorsal horn to the lateral reticular nucleus of the medulla oblongata of the rat, and the morphological types of spinal cord lamina I neurons involved were studied after injecting the retrograde tracer cholera toxin subunit B in the caudal portion of the lateral reticular nucleus. Only injection sites located in the(More)
Retrogradely labelled lamina I neurons were studied after intramesencephalic injections of subunit B of cholera toxin. The tracer was visualized with a mixture of two monoclonal antibodies followed by the peroxidase-antiperoxidase technique that produced Golgi-like staining of the labelled cells. A morphological and morphometric analysis in the three(More)
A marked expression of the c-fos proto-oncogene has been recently reported in cells of the anterior lobe of the pituitary gland in rats subject to electroacupuncture or noxious thermal stimulation under pentobarbital anaesthesia. The present study was undertaken to identify the activated pituitary cells. Following both kinds of stimulation, most(More)
The structural types of spinal cord marginal (lamina I) neurons projecting to the nucleus of the tractus solitarius (NTS) were studied. Upon injections of cholera toxin subunit B (CTb) into the caudal part of the NTS, including its lateral and medial portions, labeled cells occurred bilaterally in laminae I, IV-VII, and X, and the lateral spinal nucleus(More)
The laminar distribution of spinal cord neurones expressing immunoreactivity to the Fos protein was evaluated in the rat following chemical, thermal or mechanical noxious stimulation of the skin for 2 h. After stimulation by 20% or 5% formalin, Fos-immunoreactive neurones prevailed in lamina I where they accounted for 64% and 59%, respectively, of the(More)