Ali Akbar Sobhi Afshar

Learn More
The neural crest is a uniquely vertebrate cell type that gives rise to much of the craniofacial skeleton, pigment cells and peripheral nervous system, yet its specification and diversification during embryogenesis are poorly understood. Zebrafish homozygous for the lockjaw (low) mutation show defects in all of these derivatives and we show that low (allelic(More)
MicroRNAs (miRNAs) have attracted a great deal of attention in biology and medicine. It has been hypothesized that miRNAs interact with transcription factors (TFs) in a coordinated fashion to play key roles in regulating signaling and transcriptional pathways and in achieving robust gene regulation. Here, we propose a novel integrative computational method(More)
The full length sequence for the human pathogen coxsackievirus B6 (CVB6, Schmitt strain) has been determined. We used long RT-PCR to generate full length DNA amplicon of CVB6, and then directly sequenced the amplicons. One-step cloning of the full length amplicon enabled us to obtain an infectious clone of CVB6. RNA generated from CVB6 amplicon DNA or CVB6(More)
The goal of this study was to engineer gastrin-producing G cells of the gastric antrum to produce insulin. A pGas-Ins chimeric gene in which the gastrin promoter drives expression of the human insulin gene was constructed and was validated by transient transfection of GH4 and AGS cells. RT-PCR analysis and sequencing revealed three forms of differentially(More)
Context-dependent services are triggered when the context satisfies an execution condition. To deliver these services, user’s contexts have to be acquired by terminals such as GPS. However this service has problem of efficiency that it is necessary to collect as many contexts as possible to provide services appropriately, and problem of privacy that the all(More)
The patient described in this case had medical issues that increased her risk for restoration failure. She also had long been disappointed with her smile and the overall appearance of her teeth. The goal of managing risks and achieving improved quality of life with an esthetic and functional restoration involved preventive strategies, use of a transitional(More)
In this case involving a 29-year-old woman with a history of stomach-acid-related reflux and extensive loss of tooth structure, the patient desired to regain the smile she "used to have." The treatment goals throughout the process were to manage risk, use minimally invasive procedures, and improve the prognosis in each of the four dental(More)