Learn More
1. Evidence is summarized for the constancy of DNA content for each set of chromosomes in the various cells of an organism. 2. The DNA contents of the egg and sperm nuclei are the same. 3. A brief survey is given of DNA contents per cell in invertebrates and vertebrates. (a) In invertebrates there is some slight evidence that when primitive and higher forms(More)
17 Ohno, S., and B. M. Cattanach, Cytogenet., 1, 129 (1962). 18 Russell, L. B., Science, 140, 976 (1963). 19 Lyon, M. F., Genetic Res., 4, 93 (1963). *' Benirschke, K., R. J. Low, L. E. Brownhill, L. B. Caday, and J. deVenecia-Fernandez, Chromosoma, in press. 21 Davidson, R. G., H. M. Nitowsky, and B. Childs, these PROCEEDINGS, 50, 481 (1963). 22Beutler,(More)
The Reversibility of Coagulation It has already been shown that the coagulation of hemoglobin appears to be reversible. One can prepare from completely coagulated hemoglobin soluble, crystalline hemoglobin which by the tests so far tried is indistinguishable from ordinary native hemoglobin (Mirsky and Mason (13)). Since hemoglobin is a typical coagulable(More)
The possibility of using the Feulgen nucleal reaction for a quantitative cytochemical estimation of desoxyribonucleic acid (DNA) was investigated. The intensity of the reaction in nuclei was determined by absorption measurements with the microscope. The accuracy of such measurements was tested by comparison with measurements on the same material with a(More)
The chromatin threads, which we described briefly several years ago (1), have since then been studied carefully both morphologically and chemically. We now consider these microscopic threads of chromatin to be isolated chromosomes (2). From certain animal cells such masses of isolated chromosomes can be prepared that, with respect to quantity of material(More)
In a denatured but unhydrolyzed protein the number of SH and S-S groups detectable is equivalent to the quantity of cysteine and cystine found in the hydrolyzed protein (Mirsky and Anson, 1934-35).t Detectable protein SH groups react with iodoacetate or with oxidizing agents such as ferricyanide and cystine. Detectable protein S-S groups have the property(More)
that p is positive. However, the case of a negative p may be reduced to the case of a positive p, as seen from (6) by writing v + 1/27 for v. In this paper a structural theory of protein denaturation and coagulation is presented. Since denaturation is a fundamental property of a large group of proteins, a theory of denaturation is essentially a general(More)