Alfonso Iovieno

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Acanthamoeba keratitis (AK) is a rare but sight-threatening ocular infection. Outbreaks have been associated with contaminated water and contact lens wear. The epidemiology and pathology may be associated with unique genotypes. We determined the Rns genotype for 37 clinical isolates from 23 patients presenting at the University of Miami Bascom Palmer Eye(More)
OBJECTIVE To study the epidemiology, clinical observations, and microbiologic characteristics of fungal keratitis at tertiary eye care centers in the United States. DESIGN Retrospective multicenter case series. PARTICIPANTS Fungal keratitis cases presenting to participating tertiary eye care centers. METHODS Charts were reviewed for all fungal(More)
PURPOSE Ocular surface (OS) microbiota contributes to infectious and autoimmune diseases of the eye. Comprehensive analysis of microbial diversity at the OS has been impossible because of the limitations of conventional cultivation techniques. This pilot study aimed to explore true diversity of human OS microbiota using DNA sequencing-based detection and(More)
OBJECTIVE Fungal keratitis is a serious ocular infection that is considered to be rare among contact lens wearers. The recent Fusarium keratitis outbreak raised questions regarding the background rate of Fusarium-related keratitis and other fungal keratitis in this population. DESIGN Retrospective, multicenter case series. PARTICIPANTS Six hundred(More)
PURPOSE To determine the presence of 4 clinically relevant bacterial endosymbionts in Acanthamoeba isolates obtained from patients with Acanthamoeba keratitis (AK) and the possible contribution of endosymbionts to the pathogenesis of AK. DESIGN Experimental study. PARTICIPANTS Acanthamoeba isolates (N = 37) recovered from the cornea and contact lens(More)
PURPOSE To investigate the expression of toll-like receptors (TLRs) in healthy and active vernal keratoconjunctivitis (VKC) conjunctiva. DESIGN Comparative nonrandomized interventional study. PARTICIPANTS Nine patients with vernal keratoconjunctivitis and 10 healthy subjects. METHODS Normal and VKC conjunctiva were evaluated for TLR-2, TLR-4, and(More)
PURPOSE To evaluate and compare the depth of corneal stromal demarcation line after accelerated collagen crosslinking (CXL) using continuous and pulsed light ultraviolet-A (UVA) exposure. SETTING Department of Ophthalmology, Arcispedale Santa Maria Nuova, Reggio Emilia, Italy. DESIGN Retrospective case series. METHODS Patients with progressive(More)
PURPOSE OF REVIEW Presence and interplay of microbial flora at the ocular surface reveal dynamic and evolving interactions with implications for both ocular surface health and disease. Data in this area are scarce or non-existent. The purpose of this review is to provide a snapshot of new and emerging developments in this area over the last 12 months. (More)
Collagen cross-linking (CXL) is being offered to a growing number of keratoconic patients worldwide for prevention of disease progression, with an overall success rate that exceeds 90% of the treated eyes.1,2 However, some patients do continue to progress after CXL and may need to undergo keratoplasty. Deep anterior lamellar keratoplasty (DALK) has become(More)
PURPOSE To evaluate the efficacy of phototherapeutic keratectomy (PTK) combined with implantation of a single inferior intrastromal corneal ring segment (ICRS) and riboflavin with ultraviolet-A collagen crosslinking (CXL) performed sequentially on the same day in the management of keratoconus. SETTING Cornea Unit, Toronto Western Hospital, Toronto,(More)