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Virions of Barley stripe mosaic virus (BSMV) were neglected for more than thirty years after their basic properties were determined. In this paper, the physicochemical characteristics of BSMV virions and virion-derived viral capsid protein (CP) were analyzed, namely, the absorption and intrinsic fluorescence spectra, circular dichroism spectra, differential(More)
A series of DNA aptamers was described for human thrombin, for which the structure–function relationn ship has not been studied yet. In this work, we studied the ability of DNA aptamers of the second generation to form a GGquadruplex. The method of circular dichroism showed that aptamer 31TGT contains a GGquadruplex that is similar in structure and thermal(More)
The applicability of reversed-phase high-performance liquid chromatography (HPLC) to the analysis of the products of recombinant insulin was studied. The influence of several mobile phases in reversed-phase and ion-pair HPLC on selectivity, resolution and sensitivity was investigated. Optimum conditions for the separation of insulin-related proteins on(More)
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