Alexey Yu. Lupatov

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We studied the effect of systemic administration of multipotent stem cells on impaired neurological status in rats with brain injury. It was found that transplantation of multipotent mesenchymal stromal cells of the bone marrow or human neural stem and progenitor cells to rats with local brain injury promoted recovery of the brain control over locomotor(More)
Cultures of multipotent mesenchymal stromal cells from the pulp of human deciduous teeth (SHED cells) were characterized. The cells were used for population of 3D biodegradable polylactoglycolide scaffolds; their osteogenic potential was preserved under these conditions. Implantation of the scaffolds to mice induced no negative reactions in the recipients.(More)
The expression of puitative surface molecular markers of cancer stem cells on human colorectal adenocarcinoma cells was analyzed by flow cytofluorometry. Cell subpopulations expressing markers of epithelial and malignant cells and stem cell markers were identified. Four minor subpopulations with CD24+/CD133+, CD44+/CD133+, CD90+/CD71+, or CD90+/CD24+(More)
Comparative analysis of the expression of some surface markers of human bone marrow mesenchymal stem cells, umbilical fibroblast-like cells, and skin fibroblasts was carried out by the flow cytofluorometry method. Mesenchymal stem cells and umbilical fibroblast-like cells were similar by the levels of expression of the main histocompatibility complex(More)
Cultures of mesenchymal cells from human deciduous tooth pulp were derived. The phenotype and capacity to osteogenic and adipogenic differentiation of these cells are close to those of bone marrow mesenchymal stem cells. Deciduous tooth pulp mesenchymal cells populate biodegradable polylactide scaffolds and hence, can be used for the creation of tissue(More)
We studied immunoregulatory properties of cultured human stem cells of mesenchymal and ectodermal origins after their administration to mice. Xenotransplantation of mesenchymal stem cells from human placenta reduced the number of CD11c+ dendritic cells in mouse spleens, but did not affect activation of dendritic cells from mouse spleen in culture. It was(More)
Expression of 20 surface markers was analyzed in cultures of mesenchymal stromal cells of the umbilical cord, fibroblasts from adult and fetal human skin, and fi broblast-like cells of fetal liver was analyzed by fl ow cytometry. The studied cultures did not express hemopoietic cells markers, but were positive for CD73, CD90, and CD105 markers recommended(More)
Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per(More)
Dendritic cells (DCs) vaccination is a promising way to contend cancer metastases especially in the case of immunogenic tumors. Unfortunately, it is only rarely possible to achieve a satisfactory clinical outcome in the majority of patients treated with a particular DC vaccine. Apparently, DC vaccination can be successful with certain combinations of(More)
This concise review provides an assessment of one of the most conceptually and practically important properties of mesenchymal stromal cells, their ability to modulate immune responses, including underlying cellular and molecular mechanisms and prospects of clinical application in the treatment of autoimmune and other immunological disorders.