Alexey Novikov

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A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screening for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clones expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were capable of degrading Avicel. Clones(More)
Several numerical algorithms for dynamic Monte Carlo simulations of surface chemistry have been proposed in the past. The variable step size method (VSSM) is commonly used for systems where the rate coefficients are constant in time, owing to its good efficiency. If rate coefficients vary in time, the first reaction method (FRM) has been shown to be more(More)
A series of VLBI observations for the NOZOMI spacecraft was performed between the end of 2002 and July 2003 with aim of supporting orbit determination. We have made an astrometric analysis of the VLBI data for celestial coordinates estimation of the spacecraft with phase delay observables. Phase delay has the potential to give 3-4 orders of improvement in(More)
The plasmid pBINS1, containing the promoter, SD and leader peptide sequences of Bacillus amyloliquefaciens alpha-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of protease-deficient Bacillus subtilis AJ73 cells. The recombinant(More)
Two inexpensive inhibitors non-toxic to B. subtilis cells, O,O-diethyl-1-(N-α-hydrohexafluoro-isobutyryl)amino-1-methyl-propylphosphonate and O,O-diísobutyl-1-[2-(ethoxycarbonyl)amino-perfluoroprop-2-yl]-1-methylpropylphosphonate, when added to the culture broth, are able to prevent the proteolytic degradation of recombinant human proinsulin, secreted byB.(More)
A finite one-dimensional microscopic model of a superfluid is presented. The model consists of interacting Bose particles with an additional impurity particle confined to a ring. Both semiclassical and exact quantum calculations reveal dissipationless motion of impurity with increased effective mass due to its interaction with the excitations of Bose fluid.(More)
Resonant dielectric nanostructures represent a promising platform for light manipulation at the nanoscale. In this paper, we describe an active photonic system based on Ge(Si) quantum dots coupled to silicon nanodisks. We show that Mie resonances govern the enhancement of the photoluminescent signal from embedded quantum dots due to a good spatial overlap(More)
Rapid and sensitive methods for immunochemical estimation of bacterially-made human leukocytic interferons were developed. A large number of strains producing such interferons was analyzed with these methods and optimal ones capable of synthesizing up to 10(10), 10(8), 10(9) and 10(9) IU/l of interferons alpha A, alpha N, alpha F and alpha I1 respectively(More)
The chemically synthesized structure gene of human proinsulin was cloned in E. coli on the secretory vector containing regulatory elements of the Bacillus amyloliquefaciens alpha-amylase gene. The proinsulin gene was inserted by the EcoRI site located immediately after the DNA area encoding the alpha-amylase signal peptide. The E. coli cells transformed by(More)
Influence of O,O,-diethyl-1-(N-alpha-hydrohexafluoroisobutyryl)amino-1- methylpropylphosphonate and O,O-diisobutyl-1-[2-(ethoxycarbonyl)aminoperfluoroprop-2-yl] -1- methylpropylphosphonate on the level of production of human proinsulin secreted by a genetically engineered culture Bacillus subtilis AJ 73 (pBINS1.0.) has been studied. The above phosphonates,(More)
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