Alexandre Gauthier

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Uncontrolled proteolysis by neutrophil serine proteases (NSPs) in lung secretions is a hallmark of cystic fibrosis (CF). We have shown that the active neutrophil elastase, protease 3, and cathepsin G in CF sputum resist inhibition in part by exogenous protease inhibitors. This resistance may be due to their binding to neutrophil extracellular traps (NETs)(More)
Proteinase 3 (Pr3), the main target of anti-neutrophil cytoplasmic antibodies, is a neutrophil serine protease that may be constitutively expressed at the surface of quiescent circulating neutrophils. This raises the question of the simultaneous presence in the circulation of constitutive membrane-bound Pr3 (mPr3) and its plasma inhibitor alpha1-protease(More)
EPI-hNE4 (depelstat) is a potent inhibitor of human neutrophil elastase derived from human inter-alpha-trypsin inhibitor and designed to control the excess proteolytic activity in the sputum of cystic fibrosis patients. We analyzed its resistance to the proteolysis it is likely to encounter at inflammatory sites in vivo. EPI-hNE4 resisted hydrolysis by(More)
A lock-in amplifier is a versatile instrument frequently used in physics research. However, many students struggle with the basic operating principles of a lock-in amplifier which can lead to a variety of difficulties. To improve students’ understanding, we have been developing and evaluating a research-based tutorial which makes use of a computer(More)
1 INSERM U618 « Protéases et Vectorisation Pulmonaires » ; Université François Rabelais de Tours. 2 CHRU de Tours ; 3 INSERM U921 « Nutrition, Croissance et Cancer » ; 4 Institut Fédératif de Recherche 135 ; * Current address: Department of Neuroimmunology, Max Planck-Institute of Neurobiology, D-82152, Planegg-Martinsried, Germany Running head:(More)
We apply machine learning techniques to the problem of galaxy morphology classification. We use both supervised and unsupervised methods to study the Galaxy Zoo dataset of 61,578 pre-classified galaxies. After image pre-processing, we perform multi-class classification using the following algorithms: one-vs-all SVM with an RBF kernel, decision tree, random(More)
We describe two serious Trametes polyzona pulmonary infections, which occurred in Réunion Island, in critically ill patients. The identification was performed using sequencing of the internal transcribed spacer region of ribosomal DNA and D1/D2 region of 28S rDNA. In one case, the significance of T. polyzona in the pathological process was certain, proven(More)
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