Alexandr B. Pupyshev

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Threefold administration of benz(a)pyrene in a dose of 20 mg/kg markedly stimulated biliary excretion of lysosomal enzymes from rat liver without signs of lysosomal damage. Constant light exposure induced changes attesting to functional activation of the lysosomal apparatus in liver cells and inhibited constitutive biliary excretion of lysosomal enzymes.(More)
Whole-body controlled hyperthermia (up to 43.5 degrees C) of Wistar rats with Walker-256 carcinosarcoma was followed by symptoms of enhanced lipid peroxidation for 3-14 days and release of lysosomal enzymes into blood on day 14. Our data, in totality, point to a potential to stimulate tumor cell apoptosis.
Effects of single and repeated injections of lysosomotropic agent chloroquine on lysosomal proteolytic activity and physico-chemical properties of rat liver lysosomes have been studied. Chloroquine was administered intraperitoneally to rats at a dose of 30 mg/kg of body mass. Osmotic properties, lysosomal enzymes activity and functional state of the system(More)
Suramin that accumulates in rat liver Kupffer cell lysosomes and inhibits the intralysosomal proteolysis was used to suppress the functional activity of these particles during liver damage (acute CCl4 hepatitis). Polyvinylpyrrolidone that does not disturb protein catabolism in liver lysosomes was employed for reference. According to the characteristic(More)
Lysosomotropic agents--Triton WR 1339 and suramin--are taken up selectively into lysosomes during in vivo administration and cause specific changes of the particles. Overloading of rat liver lysosomes by Triton WR 1339 was accompanied by the labilization of lysosomes and an increased uptake of [14C]-bovine serum albumin ([14C]-BSA) by the rat liver. The(More)
Polychemotherapy with a complex of cytostatics (cyclophosphamide, doxorubicin, vincristine, prednisolone) induces progressive damage to hepatocyte membranes, which manifested in labilization of lysosomes and activation of lysosomal enzymes and serum transaminases. Enterosgel stabilized liver lysosomes and reduced manifestation of hepatocyte cytolysis.
Molecular regulation of reparative/homeostatic autophagy induced by failure of vital resources and by cellular stress is considered. Extensive autophagy regulatory apparatus responds to starvation, insufficiency of growth factors and energy supply, accumulation of unfolded proteins (ER stress), reactive oxygen species, microbial invasion. Central sensor of(More)
Light and ultrastructural morphometries were used to evaluate the effects of dextran-conjugated isoniazid (MW 30-40 kDa) on the morphology of chronic pulmonary tuberculous inflammation induced by BCG vaccine in mice. The revealed antifibrotic effect of the conjugated drug is accompanied by much less damage to the alveolar macrophageal ultrastructures (the(More)
The fluorescence technique was used to determine the effective albumin concentration (EAC) and albumin binding reserve (ABR) in 130 patients with acute viral hepatitis A and B in order to establish their values in the evaluation of endogenous intoxication (EI). The most considerable EAC and ABR reduction was found in patients with previous comorbidity and(More)