Transposons are mobile DNA segments that can disrupt gene function by inserting in or near genes. Here, we show that insertional mutagenesis by the PiggyBac transposon can be used for cancer gene discovery in mice. PiggyBac transposition in genetically engineered transposon-transposase mice induced cancers whose type (hematopoietic versus solid) and latency… (More)
We show that BRAF(V600E) initiates an alternative pathway to colorectal cancer (CRC), which progresses through a hyperplasia/adenoma/carcinoma sequence. This pathway underlies significant subsets of CRCs with distinctive pathomorphologic/genetic/epidemiologic/clinical characteristics. Genetic and functional analyses in mice revealed a series of… (More)
KRAS is one of the most frequently mutated oncogenes in human cancer. Despite substantial efforts, no clinically applicable strategy has yet been developed to effectively treat KRAS-mutant tumors. Here, we perform a cell-line-based screen and identify strong synergistic interactions between cell-cycle checkpoint-abrogating Chk1- and MK2 inhibitors,… (More)
Mouse transgenesis has provided fundamental insights into pancreatic cancer, but is limited by the long duration of allele/model generation. Here we show transfection-based multiplexed delivery of CRISPR/Cas9 to the pancreas of adult mice, allowing simultaneous editing of multiple gene sets in individual cells. We use the method to induce pancreatic cancer… (More)
This work investigates several novel variations of the Radial Basis Function Network with a view to determining whether accuracy can be maintained or improved in large dimensional classification problems without increasing amount of training data.
This work investigates combining reinforcement learning and radial basis function neural network (RBFNN) learning to improve the performance of a robot that learns to perform its task. A simple task is given, and the simulator uses reinforcement learning to generate a q-learning table, which is then used to generate a neural network. Analysis is then done… (More)
This work develops a set of instructional materials that can be used to teach basic programming concepts to high school students. The materials are tested in a series of on-campus workshops in which undergraduate students lead groups of local high school students in programming a game using Snap!. An independent evaluator assesses the effectiveness of the… (More)