Alexander Morley

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The micronucleus technique has been proposed as a method for measurement of chromosomal damage in mitogen-stimulated human lymphocytes. Micronuclei require one cell division to be expressed and, consequently, the conventional micronucleus technique is very imprecise since the cells which have undergone only one division, and the micronuclei in them, cannot(More)
Almost all of the data on the biological effects of ionizing radiation come from studies of high doses. However, the human population is unlikely to be exposed to such doses. Regulatory limits for radiation exposure are based on the linear no-threshold model, which predicts that the relationship between biological effects and radiation dose is linear, and(More)
AIMS To develop a simple, optimised, polymerase chain reaction (PCR) based method for detecting the rearranged immunoglobulin heavy chain (IgH). METHODS Using as primers oligonucleotides (Fr2A, Fr2B) homologous to the conserved sequences to the framework II region and the joining (JH) region, 25 patients with B cell lymphoproliferative disorders,(More)
The spontaneous micronucleus yield in lymphocyte cultures from healthy donors aged 0-82 years was estimated at 72 h and 96 h of culture. At both 72 and 96 h there was a positive correlation of micronucleus expression with increasing age (p less than 0.001), with an approximately 4-fold increase in micronuclei in cultures from 80-year-old donors when(More)
An animal model of chronic aplastic marlarity. Nevertheless, by 240 days 80% of row failure was produced by administerthe mice had become ill and died. Sacrifice ing busulfan to mice in high dosage for a prior to death showed that aplasia of the prolonged period. Mice which survived 60 marrow and cytopenia ( most often panor more days following cessation of(More)
A method has been developed to use the polymerase chain reaction to amplify and sequence the chain determining region 3 (CDR 3) of the human immunoglobulin heavy-chain gene, and to use the sequence as a marker for rare neoplastic B lymphocytes. Consensus primers for the Variable and Joining regions of the gene were constructed and shown to enable efficient(More)
We describe a general method to quantitate the total number of initial targets present in a sample using limiting dilution, PCR and Poisson statistics. The DNA target for the PCR was the rearranged immunoglobulin heavy chain (IgH) gene derived from a leukemic clone that was quantitated against a background of excess rearranged IgH genes from normal(More)
Serious incidents of pyrrolizidine alkaloidosis of cattle in 10 herds exposed to the Australian native plant, Senecio lautus (Asteraceae), were seen in central Queensland during 1988-1992. The deaths of 226 cattle were recorded. A mean of 8% of cattle died in affected groups (range 2 to 58%). Sickness and deaths usually occurred some months after access to(More)
Whole chromosomes within micronuclei (MN) in cytokinesis-blocked human lymphocytes were detected by using anti-kinetochore antibodies obtained from the serum of scleroderma patients. The primary antibody was localized using a peroxidase-labelled second antibody followed by a nickel chloride modification of the diamino-benzidine reaction to give a permanent(More)