Alexander L. Yudin

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Amoebae Culture Collection at the Institute of Cytology RAS in St. Petersburg (Russia) – ACCIC (SPb) – was founded in 1960; it includes strains (clones) of large free living freshwater amoebae of Amoeba proteus-type (family Amoebidae). These protists have been extensively used as model organisms in cell biology for almost two centuries. The amoebae strains(More)
The effect of cultivation temperature on differentiation of exconjugant clones for their mating types was studied in the ciliate Dileptus anser. Crosses of the clone # 7C (mating type I, MT I) with the clone # 155 (MT III) yielded 26 exconjugant F1 clones, one clone from each conjugant pair. Each young exconjugant clone was divided into two subclones. One(More)
An optical tomography investigation of the nuclear cycle in large freshwater amoebae Amoeba proteus has been performed for the first time. Nuclei of cells from a synchronized culture were stained with DAPI and examined using a confocal laser scanning microscope. Detailed analysis of three-dimensional images of the intranuclear chromatin at different stages(More)
We have obtained the first data on inheritance and genetic determination of mating types (MTs) in the lower ciliate Dileptus anser (=D. margaritifer). Observation of MT instability in young, just matured exconjugant clones obtained from some (not all) crosses appears to be the key finding in our study. In a clone of this kind, the states of maturity and(More)
The regularities of formation and electronic characteristic of photolytic silver on the surface of sols and microcrystals (MC) AgBr of various sizes and habits, as well primitive, as subjected to chemical sensitization was explored by transmission electronic microscopy (TEM) and x-ray photoelectron spectroscopy (XPS) methods. XPSresults were compared to(More)
The effects of actinomycin D on the expression and inheritance of mating types (MTs) were studied in mature laboratory clones of the ciliate Dileptus anser. In these ciliates, each mature clone isolated from the natural population belongs to one of three complementary MTs, i.e., MT I, MT II, or MT III. In the course of further cultivation of the clone under(More)
Earlier, it has been shown that some amino acids and their derivatives are able to regulate activities of adenylyl cyclase (AC) and guanylyl cyclase (GC) in free-living infusoria Dileptus anser and Tetrahymena pyriformis. The goal of this work consisted in studying the molecular mechanisms of action of methionine, tyrosine, alanine, and neurohormone(More)