the 60 main eukaryotic lineages.
BACKGROUND Recent phylogenomic analyses have revolutionized our view of eukaryote evolution by revealing unexpected relationships between and within the eukaryotic supergroups. However, for several groups of uncultivable protists, only the ribosomal RNA genes and a handful of proteins are available, often leading to unresolved evolutionary relationships. A… (More)
Cochliopodium is a very distinctive genus of discoid amoebae covered by a dorsal tectum of carbohydrate microscales. Its phylogenetic position is unclear, since although sharing many features with naked "gymnamoebae", the tectum sets it apart. We sequenced 18S ribosomal RNA genes from three Cochliopodium species (minus, spiniferum and Cochliopodium sp., a… (More)
Amoebozoa is a key phylum for eukaryote phylogeny and evolutionary history, but its phylogenetic validity has been questioned since included species are very diverse: amoebo-flagellate slime-moulds, naked and testate amoebae, and some flagellates. 18S rRNA gene trees have not firmly established its internal topology. To rectify this we sequenced cDNA… (More)
Sequence-Specific Amplified Polymorphism (S-SAP)-analysis of 131 accessions of different species from five sections of the genus Malus was carried out to study genetic diversity, and to clarify phylogenetic and taxonomic issues. S-SAP-markers, based on long terminal repeat (LTR)-retrotransposons TRIM2 and dem1, were developed, which identified 679… (More)
I have re-investigated the light-microscopic features of Parvamoeba rugata Rogerson, 1993, type strain CCAP 1556/1. The major characters of amoebae correspond to the initial description of this species. However, one peculiarity demonstrated by the cells during adhesion to the substratum, seems to have been partly underestimated previously. At the same time… (More)
Cochliopodium gallicum n. sp., isolated from cyanobacterial mats in the Camargue (France) is the smallest marine species of Cochliopodium to date. Its unusual tectum consists of flat plate-shaped scales with honeycomb-like centres, underlain by a layer of filamentous structures connected to each other in the basal and apical parts. The tectum is very fine… (More)