Alexander Grünberger

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The majority of biotechnologically relevant metabolites do not impart a conspicuous phenotype to the producing cell. Consequently, the analysis of microbial metabolite production is still dominated by bulk techniques, which may obscure significant variation at the single-cell level. In this study, we have applied the recently developed Lrp-biosensor for(More)
In this protocol the fabrication, experimental setup and basic operation of the recently introduced microfluidic picoliter bioreactor (PLBR) is described in detail. The PLBR can be utilized for the analysis of single bacteria and microcolonies to investigate biotechnological and microbiological related questions concerning, e.g. cell growth, morphology,(More)
Microfluidics has become an essential tool in single-cell analysis assays for gaining more accurate insights into cell behavior. Various microfluidics methods have been introduced facilitating single-cell analysis of a broad range of cell types. However, the study of prokaryotic cells such as Escherichia coli and others still faces the challenge of(More)
MOTIVATION Single cell time-lapse microscopy is a powerful method for investigating heterogeneous cell behavior. Advances in microfluidic lab-on-a-chip technologies and live-cell imaging render the parallel observation of the development of individual cells in hundreds of populations possible. While image analysis tools are available for cell detection and(More)
Phase contrast microscopy cannot give sufficient information on bacterial metabolic activity, or if a cell is dead, it has the fate to die or it is in a viable but non-growing state. Thus, a reliable sensing of the metabolic activity helps to distinguish different categories of viability. We present a non-invasive instantaneous sensing method using a(More)
Recombinant protein production is mostly realized with large-scale cultivations and monitored at the level of the entire population. Detailed knowledge of cell-to-cell variations with respect to cellular growth and product formation is limited, even though phenotypic heterogeneity may distinctly hamper overall production yields, especially for toxic or(More)
BACKGROUND Microfluidic lab-on-chip technology combined with live-cell imaging has enabled the observation of single cells in their spatio-temporal context. The mother machine (MM) cultivation system is particularly attractive for the long-term investigation of rod-shaped bacteria since it facilitates continuous cultivation and observation of individual(More)
Literatur [1] Grünberger A, Wiechert W, Kohlheyer D (2014) Single-cell microfluidics: opportunity for bioprocess development. A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level. growth rate 0.6: what drives Corynebacterium glutamicum to higher growth rates in defined medium.(More)
Photocaged compounds are light-responsive biomolecules that regain their primal function upon short light exposure. Thus, cellular functions such as bacterial gene expression can be non-invasively triggered in a gradual and homogenous fashion by light. Especially for single-cell research, optogenetic tools exhibit an enormous potential for achieving an(More)
A disposable microfluidic chip system for single cell analysis is presented. The device enables hydrodynamic trapping of single bacteria cells of various industrial prokaryotic strains, e.g., E. coli and C. glutamicum, in partly sub-µm fluidic structures. Single cell traps are arranged in arrays aiming for quasi high-throughput cell observation with respect(More)
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