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Combining intracellular recording and dye-injection techniques permitted direct correlation of neuronal soma size with peripheral nerve conduction velocity in individual neurones of the L4 dorsal root ganglion (d.r.g.) of the anaesthetized 5-8-week-old rat. The conduction velocities fell into two main groups; those greater than 14 m/s (A alpha and beta(More)
The electrical characteristics of isolated neonatal rat intracardiac neurons were examined at 22 and 37 degrees C using the perforated-patch whole cell recording technique. The mean resting membrane potential was -52.0 mV at 37 degrees C and exhibited no temperature dependence. Lowering the temperature from 37 to 22 degrees C decreased the mean input(More)
A monoclonal antibody (RT97) against neurofilament protein specifically and exclusively labelled a subpopulation of rat dorsal root ganglion (DRG) neurones. For seven ganglia (L4 and T13) studied quantitatively the frequency distribution histograms of the size of labelled cells could be fitted by a single normal distribution whose parameters were extremely(More)
We charted postnatal changes in the intrinsic electrophysiological properties and synaptic responses of rat intrinsic cardiac ganglion (ICG) neurons. We developed a whole-mount ganglion preparation of the excised right atrial ganglion plexus. Using intracellular recordings and nerve stimulation we tested the hypothesis that substantial transformations in(More)
The hyperpolarization-activated nonselective cation current, I(h), was investigated in neonatal and adult rat intracardiac neurons. I(h) was observed in all neurons studied and displayed slow time-dependent rectification. I(h) was isolated by blockade with external Cs(+) (2 mM) and was inhibited irreversibly by the bradycardic agent, ZD 7288. Current(More)
The electrical characteristics of individual rat dorsal root ganglion neurones were studied and related to the peripheral axon conduction velocity and morphological cell type. Neurones were divided into four groups based on the conduction velocity of their peripheral axons (A alpha, 30-55 m/s; A beta, 14-30 m/s; A delta, 2.2-8 m/s and C less than 1.4 m/s).(More)
1. The actions of high hydrostatic pressure (10.4, 20.8 MPa) on the membrane currents of Helix neurones were examined under voltage clamp. 2. High hydrostatic pressure (20.8 MPa) reduced the maximum inward current to 0.78 and the delayed outward current, measured at the inward current reversal potential, to 0.75 of their value at atmospheric pressure. 3.(More)
The electrical properties of the somata of neurones associated with low-threshold, cutaneous mechanoreceptors were studied in young, anaesthetized rats. The adaptation properties of slowly and rapidly adapting afferents were compared by recording from the somata during mechanical stimulation of the skin and during the injection of depolarizing current(More)
Monophasic compound action potentials were recorded from Rana sciatic nerves. Three distinct peaks were observed and designated A alpha, A delta and C. All peaks were abolished by replacement of the external medium with Na(+)-free solution. However, the C peak alone was unaffected by external application of 1 microM tetrodotoxin (TTX), both A peaks were(More)
The effects of gamma-aminobutyric acid (GABA) on the electrophysiological properties of intracardiac neurones were investigated in the intracardiac ganglion plexus in situ and in dissociated neurones from neonatal, juvenile and adult rat hearts. Focal application of GABA evoked a depolarizing, excitatory response in both intact and dissociated intracardiac(More)