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The recruitment model for gene activation stipulates that an activator works by bringing the transcriptional machinery to the DNA. Recent experiments in bacteria and yeast indicate that many genes can be activated by this mechanism. These findings have implications for our understanding of the nature of activating regions and their targets, and for the role(More)
Withdrawal from the cell cycle is an essential aspect of vertebrate muscle differentiation and requires the retinoblastoma (Rb) protein that inhibits expression of genes needed for cell cycle entry. It was shown recently that cultured myotubes derived from the Rb-/- mouse reenter the cell cycle after serum stimulation (Schneider, J.W., W. Gu, L. Zhu, V.(More)
Cells detect extracellular signals by allostery and then give those signals meaning by 'regulated localization'. We suggest that this formulation applies to many biological processes and is particularly well illustrated by the mechanisms of gene regulation. Analysis of these mechanisms reveals that regulated localization requires simple molecular(More)
One polypeptide, designated S, confers sequence-specificity to the multisubunit type I restriction enzymes. Two families of such enzymes, K and A, include members that recognize diverse, bipartite, target sequences. The S polypeptides of the K family, while having areas of near identity, also contain two extensive regions of variable sequence. We now show(More)
Shortly after the dawn of biochemical genetics, Escherichia coli K-12 replaced Neurospora crassa as the key model organism. With E. coli K-12 came another, even simpler, system: each bacterial cell contained a dormant virus (bacteriophage) called lambda (λ). Occasionally, the quiescent λ genome was activated to generate free phage particles. Thus it was(More)
''The game was that of continually inventing a possible world, or a piece of a possible world, and then of comparing it with the real world...What mattered more than the answers were the questions and how they were formulated.'' Francois Jacob, The Statue Within. As Francois Jacob tells it, one afternoon in September 1958, just back from New York, he walked(More)