Alex Michler

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Modulation of neurite growth by gamma-aminobutyric acid (GABA) and several agonists and antagonists to its receptors was analysed in neuronal cultures of embryonic chick tectum and rat cerebellum, respectively, using morphometric methods. In each case, modulation of growth by GABA was similar in both types of neurons. However, data on neurite elongation(More)
The effects of gamma-aminobutyric acid (GABA) and related agonists and antagonists on the excitatory cell death were examined in dispersed primary cultures of the rat cerebral cortex. The cytotoxic effects evoked by kainic acid, quisqualic acid and N-methyl-D-aspartic acid were evaluated by phase contrast microscopy and quantified by the measurement of(More)
The excitotoxicity of glutamate, N-methyl-D-aspartate (NMDA), quisqualate and kainate were compared in rat cerebro-cortical cell cultures grown in different media. Excitotoxic cell death was examined by phase contrast microscopy and by the measurement of lactic dehydrogenase (LDH) activity released in the culture medium. Cells grown for 14 days in a(More)
In contrast to results of earlier investigations the influence of thyroxine on CNS connectivity is not restricted to circum-metamorphic stages in frogs. Neuroanatomical findings in adult Xenopus treated with thyroxine reveal a spread of the ipsilateral retino-tectal projection. Sprouting fibers establish a tectal innervation pattern similar to the one found(More)
The effects of gamma-aminobutyric acid (GABA) and selective agonists of GABAA and GABAB receptors were examined on excitotoxic cell death evoked in primary cultures of the rat cerebral cortex by glutamate and related excitotoxins. The activity of lactate dehydrogenase released from irreversibly damaged cells into the culture medium was taken as a measure of(More)
Deposition of latex colloids on a structured silicon surface was investigated. The surface with well-defined roughness and topography pattern served as an analogue for rough mineral surfaces with half-pores in the submicrometer size. The silicon topography consists of a regular pit pattern (pit diameter = 400 nm, pit spacing = 400 nm, pit depth = 100 nm).(More)
Neurite elongation was investigated in explanted chick dorsal root ganglia (DRG) using either serum-supplemented or defined N1 medium and different culture substrata. The results indicate that there is no substantial difference in neurite elongation on the three substrata when ganglia are grown in N1 medium. In contrast, neurite length depends on the(More)
Experiments with dissociated chick dorsal root ganglion cells cultured in different media revealed the significance of non-neuronal cells for neurite growth along a physical boundary. The evidence presented suggests that fibrillary fibronectin deposited by non-neuronal cells guides neurites, rather than the physical boundary itself. This guidance is not a(More)
The influence of gamma-aminobutyric acid (GABA) receptor agonists and antagonists on the intracellular concentration of inositol 1,4,5-triphosphate (IP3) was examined in neuronal cultures of embryonic chick tectum. GABA and selective agonists of its receptors were inactive, whereas phaclofen, a GABA-B receptor antagonist, increased IP3 levels in a(More)
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