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Large hydrogen-isotopic (D/H) fractionations between lipids and growth water have been observed in most organisms studied to date. These fractionations are generally attributed to isotope effects in the biosynthesis of lipids, and are frequently assumed to be approximately constant for the purpose of reconstructing climatic variables. Here, we report D/H(More)
The rise of atmospheric O(2) was a milestone in the history of life. Although O(2) itself is not a climate-active gas, its appearance would have removed a methane greenhouse present on the early Earth and potentially led to dramatic cooling. Moreover, by fundamentally altering the biogeochemical cycles of C, N, S and Fe, its rise first in the atmosphere and(More)
Isotopic compositions of carbon-bound hydrogen in individual compounds from eight di€erent organisms were measured using isotope-ratio-monitoring gas chromatography±mass spectrometry. This technique is capable of measuring D/H ratios at natural abundance in individual lipids yielding as little as 20 nmol of H 2 , and is applicable to a wide range of(More)
Sedimentary hopanes are pentacyclic triterpenoids that serve as biomarker proxies for bacteria and certain bacterial metabolisms, such as oxygenic photosynthesis and aerobic methanotrophy. Their parent molecules, the bacteriohopanepolyols (BHPs), have been hypothesized to be the bacterial equivalent of sterols. However, the actual function of BHPs in(More)
The extent to which leaf water D-enrichment (transpiration) and soil water D-enrichment (evaporation) affect the D/H ratio of plant leaf waxes remains a contentious issue, with important implications for paleohydrologic reconstructions. In this study we measure dD values of precipitation (dD p), groundwater (dD gw), plant xylem water (dD xw) and leaf water(More)
—Hydrogen isotope ratios (D/H) of lipid biomarkers extracted from aquatic sediments were measured to determine whether they can be used as a proxy for D/H of environmental water. Values of ␦D were determined by using a recently developed isotope-ratio-monitoring gas chromatograph-mass spectrometer system (irmGCMS) and were confirmed by conventional hydrogen(More)
We report the abundances and hydrogen-isotopic compositions (D/H ratios) of fatty acids extracted from hot-spring microbial mats in Yellowstone National Park. The terrestrial hydrothermal environment provides a useful system for studying D/H fractionations because the numerous microbial communities in and around the springs are visually distinct, separable,(More)
Compound-specific analyses of lipid D/H ratios often encounter ranges of 300 per thousand or more, and experiments using D-enriched water to study fractionations often extend the range up to 1000 per thousand. Here we show that for such large dynamic ranges in D/H ratio, isotopic "memory" between adjacent peaks can be significant. Memory effects have not(More)
Hopanoids are triterpenoids produced mainly by bacteria, are ubiquitous in the environment, and have many important applications as biological markers. A wide variety of related hopanoid structures exists, many of which are polyfunctionalized. These modifications render the hopanoids too involatile for conventional gas chromatography (GC) separation, so(More)
We describe a moving-wire analyzer for measuring 13C in dissolved, involatile organic materials. Liquid samples are first deposited and dried on a continuously spooling nickel wire. The residual sample is then combusted as the wire moves through a furnace, and the evolved CO2 is analyzed by continuous-flow isotope ratio mass spectrometry. A typical analysis(More)