Alex L Kahler

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highly informative DNA markers evenly spaced throughout the genome. A number of molecular genetic maps of the soybean [Glycine max Using a mapping population derived from an inter(L.) Merr.] have been developed over the past 10 yr. These maps are specific G. max 3 G. soja cross, Shoemaker and Olson primarily based on restriction fragment length polymorphism(More)
 An RFLP linkage map was constructed for cultivated sunflower Helianthus annuus L., based on 271 loci detected by 232 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. These genetic markers plus a fertility restoration gene, Rf 1, defined 20 linkage groups, covering 1164 cM of the(More)
A multilocus method of estimating mating system parameters in populations is presented that recovers information from classification over multiple loci that single-locus estimators do not detect. It is shown that the multilocus estimator provides a standard of reference (null hypothesis) that can be useful in analyzing the effects of factors such as(More)
Two populations of Avena barbata have been sampled intensively and allelic frequencies have been determined on a fine grid for six enzyme loci. The distribution of alleles among microniches within the location occupied by one of these populations (CSA-1) was correlated with environment following patterns of macro- and microdifferentiation previously(More)
An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from 199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage(More)
Twelve U.S. Corn Belt open-pollinated and five adapted exotic populations of maize (Zea mays L.) were assayed for allozyme (allele) variation at 13 enzyme marker loci. Extensive allozyme variability was observed in all populations studied. No locus was monomorphic over all populations. Each of the lociIdh2, Got1, Mdh2, Pgd1, andPgd2 expressed two allozymes(More)
Changes in gene and genotypic frequencies at four esterase loci were monitored over 25 generations in Composite Cross V, an experimental population of barley, to obtain experimental evidence concerning the balance of forces responsible for: (1) the marked differences in allelic frequencies among barleys from different ecogeographical regions of the world;(More)
The dynamics of gametic frequency change have been analyzed in two experimental plant populations. Individual plants were scored for their genotype at four enzyme loci, and four-locus joint gametic frequencies were estimated from the genotypic data over generations. Striking correlations developed in allelic state over loci, including correlations between(More)
Diagnostic methods were used to identify and quantify Myxobolus cerebralis, a myxozoan parasite of salmonid fish. In this study, 7-week-old, pathogen-free rainbow trout (Oncorhynchus mykiss) were experimentally infected with M. cerebralis and at 7 months postinfection were evaluated with 5 diagnostic assays: 1) pepsin-trypsin digest (PTD) to detect and(More)
Randomness of fertilization was studied in an open-pollinated population of maize (Zea mays L.) through allozyme assays of seedlings from open-pollinated seeds produced on both tasseled and detasseled plants. Mixed-mating-model estimates of the amount of outcrossing (t) were not significantly different from t = 1.00 for four enzyme loci ( Adh1, Idh2, Got1(More)