Alessia Candeo

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Selective Plane Illumination Microscopy (SPIM) is an imaging technique particularly suited for long term in-vivo analysis of transparent specimens, able to visualize small organs or entire organisms, at cellular and eventually even subcellular resolution. Here we report the application of SPIM in Calcium imaging based on Förster Resonance Energy Transfer(More)
Calcium oscillations play a role in the regulation of the development of tip-growing plant cells. Using optical microscopy, calcium oscillations have been observed in a few systems (e.g. pollen tubes, fungal hyphae, algal rhizoids). High resolution, non-phototoxic and rapid imaging methods are required to study the calcium oscillation in root hairs. We show(More)
Polyplexes are nanoparticles formed by the self-assembly of DNA/RNA and cationic polymers specifically designed to deliver exogenous genetic material to cells by a process called transfection. There is a general consensus that a subtle balance between sufficient extracellular protection and intracellular release of nucleic acids is a key factor for(More)
We introduce a scheme for the generation of tunable few-optical-cycle UV pulses based on sum-frequency generation between a broadband visible pulse and a narrowband pulse ranging from the visible to the near-IR. This configuration generates broadband UV pulses tunable from 0.3 to 0.4 μm, with energy up to 1.5 μJ. By exploiting nonlinear phase transfer,(More)
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