Alessandro Negro-Ponzi

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Mouse gamma interferon (IFN-gamma) was produced from a cloned T-cell lymphoma, L12-R4, stimulated with phorbol myristic acetate (PMA) at a concentration of 2 X 10(-7) M/ml. Absorption of crude IFN-gamma on silicic acid resulted in a 100-fold concentration and 10-fold purification. After application of this IFN-gamma to a Sephacryl S-200 column, 100% of the(More)
The alloantigens encoded by the major histocompatibility complex (MHC) play a fundamental role in regulating lymphocyte activities, at both the recognitive and effector phases of the immune response. At the recognitive level, the presence or absence of some MHC antigens strongly modulates the immunogenicity of a second antigen expressed on the same cell(More)
Mouse immune interferon was produced from a T-lymphoma cell line, L12-R4, upon stimulation with phorbol myristic acetate at a concentration of 2 x 10(-7) M/ml. The crude interferon, concentrated by precipitation with (NH4)2SO4 at concentration of 80%, was sequentially chromatographed on two sorbents, namely Sephacryl S-200 and Phenyl-Sepharose CL-4B. With(More)
The characteristics of the responder and stimulating cells involved in migration inhibition factor (MIF) production in primary 'one-way' mouse mixed lymphocyte reactions (MLR) were analyzed by using an indirect agarose droplet assay. T-lymphocytes are mainly responsible for MIF release, as shown by pretreatment with anti-Thy 1.2 serum plus complement or(More)
In the present study we have analyzed the biochemical characteristics of murine immune interferon (IFN-y). Ionic or non-ionic detergents inactivate IFN-y, while as far as organic solvents are concerned only those with high polarity inhibit antiviral activity. Finally when different kinds of enzymes were used, proteases but not lipases or neuraminidase(More)
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