Alessandro Gherardi

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Vignetting is the radial attenuation effect of the image's brightness intensity from the center of the optical axis to the edges. To perform quantitative image analyses it is mandatory to take into account this effect, intrinsic of the acquisition system. Many image processing steps, such as segmentation and object tracking, are strongly affected by(More)
This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for(More)
Automated microscopic image analysis techniques are increasingly gaining attention in the field of biological imaging. The success of these applications mostly depends on the earlier image processing steps applied to the acquired images, aiming at enhancing image content while performing noise and artifacts removal. One such artifact is the vignetting(More)
The measurement in metric units from a perspective view of a real world object is an important task in many computer vision applications. The goal of the perspective calibration is to map the reference coordinates of the 3D object into the 2D image coordinates. This is usually done by recovering the perspective transformation parameters by presenting to the(More)
Many methods are known in literature to reconstruct the camera response function (RF), whether they use calibration chart or images of arbitrary scenes taken at different exposures. This works in case to have at one's disposal a camera with enough exposure steps to capture the whole dynamic range of the scene, representatively. However, in case of(More)
CONTEXT Mosaics of Whole Slides (WS) are a valuable resource for pathologists to have the whole sample available at high resolution. The WS mosaic provides pathologists with an overview of the whole sample at a glance, helping them to make a reliable diagnosis. Despite recent solutions exist for creating WS mosaics based, for instance, on automated(More)
The skin care reveals of great importance for matters of both aesthetics and health. Often, the skin appearance gives us information about the skin health status as well as hints at the age of a person. However, it has always been difficult to give a measure of the skin health status as well as of the human aging process by simply analyzing the skin surface(More)