Aldona Pieńkowska

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Karyotyping of dog chromosomes is a difficult task owing to the high diploid number of chromosomes (2n=78) and the similar morphology of autosomes, all of which are acrocentrics. In this report 22 of the 39 G-banded chromosome pairs and their corresponding ideograms have been standardized. The ideogram comprises altogether 235 bands. The need for the(More)
Three copies of the X chromosome were identified in a 5-year-old mixed breed infertile bitch. One year after the cytogenetic examination, the bitch died due to gastritis hemorrhagica, an inflammation of the mucus coat of the stomach. Dental studies showed congenital lack of some premolar and molar teeth. Ovaries were of normal shape and size. Also,(More)
We have developed a novel method for identifying dog chromosomes and unambiguously mapping specific clones onto canine chromosomes. This method uses a previously established red fox/dog comparative chromosome map to guide the FISH mapping of cloned canine DNA. Mixing metaphase preparations of the red fox and dog enabled a single hybridization to be(More)
A registered female Yorkshire terrier aged 3 years was diagnosed as intersex. Molecular and cytogenetic studies were carried out to determine whether chromosomal abnormalities were involved in the development of this condition. Analysis of the testicular tissue in the gonads and PCR amplification of three canine Y chromosome-specific DNA sequences from the(More)
The purpose of this study was to determine the nucleotide sequence of canine 5S rRNA and use this information to develop a molecular probe to assign the gene locus to chromosomes of the dog and three other related canid species using fluorescence in situ hybridization. The nucleotide sequence of canine liver 5S rRNA is 120 base pairs long and identical to(More)
Four species of the family Canidae were cytogenetically studied. The activity of NORs was detected with the use of silver staining. The number of NORs was characteristic for a given karyotype. For the dog found on autosomes 7, 17, 20 and on sex chromosome (Y), for the racoon dog on autosomes 1, 4, 13 and on sex chromosome (Y), for the silver fox only on(More)
A population of Baudet du Poitou donkeys was genetically characterized using microsatellites. The results were used to verify the pedigrees and to estimate the genetic variability. It could be confirmed that a equine parentage test kit works well for donkeys and that by using 13 microsatellites more than 99% of wrong pedigree informations would be detected.(More)
Using fluorescent in situ hybridization on Q-banded chromosomes, nucleolar organizer regions (NORs) were localized on canine chromosomes 7qter, 17qter, Yqter and also terminally on a small autosome, not yet included in the dog standard karyotype. The analysis of NOR activity was carried out on Ag-I stained metaphases originating from 27 individuals (10(More)
Each year approximately 13,000 new pedigrees for puppies are issued by the Swiss Kennel Club Except for a few cases there are no regulations to verify the paternities independently. This report describes the practical procedure for disputed paternities and summarizes the results from 35 cases which were resolved during the last three years. Most cases(More)