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Karyotyping of dog chromosomes is a difficult task owing to the high diploid number of chromosomes (2n=78) and the similar morphology of autosomes, all of which are acrocentrics. In this report 22 of the 39 G-banded chromosome pairs and their corresponding ideograms have been standardized. The ideogram comprises altogether 235 bands. The need for the… (More)
We have developed a novel method for identifying dog chromosomes and unambiguously mapping specific clones onto canine chromosomes. This method uses a previously established red fox/dog comparative chromosome map to guide the FISH mapping of cloned canine DNA. Mixing metaphase preparations of the red fox and dog enabled a single hybridization to be… (More)
A registered female Yorkshire terrier aged 3 years was diagnosed as intersex. Molecular and cytogenetic studies were carried out to determine whether chromosomal abnormalities were involved in the development of this condition. Analysis of the testicular tissue in the gonads and PCR amplification of three canine Y chromosome-specific DNA sequences from the… (More)
Three copies of the X chromosome were identified in a 5-year-old mixed breed infertile bitch. One year after the cytogenetic examination, the bitch died due to gastritis hemorrhagica, an inflammation of the mucus coat of the stomach. Dental studies showed congenital lack of some premolar and molar teeth. Ovaries were of normal shape and size. Also,… (More)
Each year approximately 13,000 new pedigrees for puppies are issued by the Swiss Kennel Club Except for a few cases there are no regulations to verify the paternities independently. This report describes the practical procedure for disputed paternities and summarizes the results from 35 cases which were resolved during the last three years. Most cases… (More)
A population of Baudet du Poitou donkeys was genetically characterized using microsatellites. The results were used to verify the pedigrees and to estimate the genetic variability. It could be confirmed that a equine parentage test kit works well for donkeys and that by using 13 microsatellites more than 99% of wrong pedigree informations would be detected.… (More)
Using fluorescent in situ hybridization on Q-banded chromosomes, nucleolar organizer regions (NORs) were localized on canine chromosomes 7qter, 17qter, Yqter and also terminally on a small autosome, not yet included in the dog standard karyotype.
Microdissection of chromosomes and subsequent amplification of the DNA material by PCR allow the development of libraries, region-specific DNA probes or chromosome painting probes. Veterinary medicine will benefit from this method in instances where clarification of numercal or structural chromosome aberrations is needed.