Alberto Piña-Escobedo

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Obesity and overweight are health problems of multifactorial etiology, which may include changes in the microbiome. In Mexico, more than 30 % of the child population between 5 and 11 years of age suffer from being overweight or are obese, which makes it a public health issue in progress. The purpose of this work was to measure the short-chain fatty acid(More)
In the present work we have used a double-hybrid assay in bacteria to identify a putative domain in E. coli PNPase required for in vivo interaction with RNase E. We used a 202 aa fragment of RNase E previously reported as the PNPase binding domain in this enzyme and a collection of 13 different fragments of 105 aa, spanning the entire sequence of 734 aa(More)
PNPase is a phosphate-dependent exonuclease of Escherichia coli required for growth in the cold. In this work we explored the effect of specific mutations in its two RNA binding domains KH and S1 on RNA binding, enzymatic activities, autoregulation and ability to grow at low temperature. We removed critical motifs that stabilize the hydrophobic core of each(More)
Greater Mexico City is one of the largest urban centers in the world, with an estimated population by 2010 of more than 20 million inhabitants. In urban areas like this, biological material is present at all atmospheric levels including live bacteria. We sampled the low atmosphere in several surveys at different points by the gravity method on LB and blood(More)
Obesity is an epidemic malady in Mexico with genetics roots in the chromosomal human genome, however nowadays it has also been recognized the role of the human microbiome in the development of this condition. Normally, the colon microbiota produces short chain fatty acids (SCFA) by fermentation of undigested carbohydrates, and changes in the bacterial(More)
The original published version of this article has some imprecisions in the coordinates for the forward and reverse primers in 16S rDNA molecule, B16S rDNA Libraries^ of the BMaterials and Methods^ section (Page no. 76). The correct version is presented below: The seven forward primers used in this work, V3-341F2, V3341F4, V3-341F7, V3-341F8, V3-341F9,(More)
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