Alberto Luaces

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We report a one-step method for the purification to homogeneity of a cysteine proteinase of Entamoeba histolytica (histolysin) by affinity chromatography of the soluble extract of the parasite on immobilized phenylalanyl(2-phenyl)aminoacetaldehyde semicarbazone. The enzyme has an apparent Mr of 26,000 by SDS/polyacrylamide-gel electrophoresis and 29,000 by(More)
Entamoeba histolytica is a common parasite of the human intestine, and in a significant percentage of cases it invades the tissues. Virulent strains of the organism produce the proteinose histolysain, which almost certainly facilitates the invasive behaviour. Histolysoin has been isolated, a selective assay developed, and specific antisera raised against(More)
An online library of computational multibody benchmark problems was presented by Masoudi et al. [3], along with its potential applications in the field and anticipated benefits to the multibody community. The web-based library was initially developed as a collaboration between themark library has since been refined and improved, applying feedback from(More)
A solid-phase enzyme immunoassay (EIA) was used to detect circulating antibodies to histolysain, the major cysteine proteinase of Entamoeba histolytica. Serum samples from 40 healthy controls, 33 asymptomatic E. histolytica cyst passers and 22 patients with amoebic liver abscess were tested. Antibodies to histolysain were found in 72.7% of cases of amoebic(More)
We describe a new, improved test for the detection of intestinal infection by Entamoeba histolytica. The test depends upon immunoadsorption of the E. histolytica cysteine proteinase, histolysain, from faecal samples, and subsequent visual detection of the enzyme by a colour reaction. With samples from 200 volunteers, results agreed closely with those(More)
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