The distance between the simian virus 40 early promoter elements has been altered by inserting either odd or even multiples of half a DNA turn. There are marked differences in the in vivo effects of these two types of insertions on initiation of transcription from this promoter.
Enhancers are cis-acting activators of transcription from homologous or heterologous promoter elements of viral and cellular genes (see refs 1-6 for reviews). The activity of the simian virus 40 (SV40) (refs 7-9) and immunoglobulin heavy-chain gene (IgH) (refs 10, 11) enhancers has been reproduced to some extent in vitro and appears to be mediated by… (More)
The large tumor antigen (TAg) of simian virus 40 regulates transcription of the viral genes. The early promoter is repressed when TAg binds to the origin and DNA replication begins, whereas the late promoter is activated by TAg through both replication-dependent and -independent mechanisms. Previously it was shown that activation is diminished when a site… (More)
Our limited understanding of the relationship between the behavior of individual neurons and large neuronal networks is an important limitation in current epilepsy research and may be one of the main causes of our inadequate ability to treat it. Addressing this problem directly via experiments is impossibly complex; thus, we have been developing and… (More)
Ma netomyography (MMG) is a new non-invasive technique fins ired by the magnetoneurographic method of Wikswo [ l f t o detect action currents in a muscle, which is immersed in a well conducting 5uid. The detection coil is of a toroidal shape, with the muscle lead through the center of the coil. For a long muscle which fits tightly in the toroid, it is to be… (More)
Stimulation of in vitro transcription by the simian virus 40 enhancer involves a rapid and stable binding of a trans-acting factor with both the 5'- and 3'-domains of the enhancer sequence. The enhancer factor, which differs from other types of transcriptional factors, can interact with other enhancer elements.