Learn More
Microtubule polymer levels in mouse 3T6 fibroblasts and primary cultures of rat hepatocytes can be manipulated by treatment of cells with long term, low doses of colcemid. Such treatment produces a rather uniform population of cells with microtubules of reduced lengths. Using this system, we demonstrate (a) that the rate of tubulin synthesis is sensitive to(More)
Researchers at the National Institute of Standards and Technology are developing a virtual manufacturing cell. This cell will contain simulation models of a wide range of manufacturing equipment, processes, and systems. It will have commercial and prototype applications software which implement production functions from order release to final inspection.(More)
The effects on tubulin messenger RNA levels and tubulin protein synthesis of treating cells with microtubule-depolymerizing drugs or directly microinjecting cells with tubulin have suggested that non-polymerized tubulin depresses its own synthesis. The precise level of this control is unclear. It has been shown that enucleated cells, termed cytoplasts,(More)
This paper proposes an integration test-bed system for supply chain management, which forms the foundation for the construction of a valued manufacturing chain. The core system of the test-bed is a hierarchical simulation system to support production management in supply chain. The test-bed possesses sub-components linked with the simulation system. These(More)
The population of the province of Newfoundland and Labrador is genetically isolated. This isolation is evidenced by an overabundance of several monogenic disorders. The Newfoundland population, like that of other isolates, is now the focus of interest for identification of genes implicated in common diseases. However, the utility of such populations for(More)
Quantitative electron microscopic autoradiography and diaminobenzidine cytochemistry provide evidence for an uptake and vesicular transport mechanism for iodine-125-labeled immunoglobulin A from plasma to bile by hepatocytes in vivo. The data confirm the existence of a hepatobiliary pathway for secretion of immunoglobulin A into the intestine and are(More)
The specific localization and the characterization of the parathyroid hormone (PTH) receptor in bone have been studied using 18-d embryonic chick calvariae and biologically active, electrolytically labeled [1251] bovine PTH(I-34). Binding was initiated by adding [12Sl]-bPTH(I-34) to bisected calvariae at 30°C. Steady state binding was achieved at 90 min at(More)
Researchers at the National Institute of Standards and Technology have been developing a simulation-based production testbed. This testbed contains continuous simulation models of production equipment and processes, and discrete-event simulation models of various production systems. In addition, it contains both commercial and prototype software(More)
We describe an architecture to validate new tools in support of interoperability provisioning processes that take place within industrial communities. We start from a best-practices standards development process where an industry community develops interoperability specifications that are used by vendors to make their software applications compliant to the(More)
Stereological analysis of hepatic fine structure in Fischer 344 male rats at 1, 6, 10, 16, 20, 25, and 30 mo of age revealed differences in the amounts and distributions of hepatocellular organelles as a function of sublobular location or animal age. Between 1 and 16 mo of age, both the centrolobular and periportal hepatocytes increased in volume by 65 and(More)